Skip to main content
SpringerLink
Log in

Interaction of free indole-3-acetic acid and its amino acid conjugates in tomato hypocotyl cultures

  • Published:
Journal of Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The interaction of free IAA and its amino acid conjugates on growth and development of cultured tomato hypocotyl tissue (Lycopersicon esculentum Mill. cv. Marglobe) was studied. In a nutrient medium containing 10 μmol/L of benzyladenine, free IAA stimulated shoot and root development with little callus proliferation. In contrast, all IAA-amino acid conjugates tested supported mostly callus growth. Simultaneous application of free IAA and its conjugates resulted in the expression of mixed morphogenetic responses (i.e., both vigorous callus growth and organogenesis resulted). Growth kinetics and the effect of temporal exposure of the tissues to the bound and the free auxin suggest that some IAA-amino acid conjugates may specifically influence plant morphogenesis in ways that cannot be easily explained as simply a function of their slow hydrolysis to release free IAA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

IAA:

indole-3-acetic acid

IAA-Ala:

N-(indol-3-ylacetyl)-l-alanine

IAA-Asp:

N-(indol-3-ylacetyl)-dl-aspartic acid

IAA-Lys:

N ε-(indol-3-ylacetyl)-l-lysine

IAA-Orn:

N δ-(indol-3-ylacetyl)-l-ornithine

IAA-Thr:

N-(indol-3-ylaцetyl)-l-threonine

References

  • Bialek K, Meudt W, Cohen JD (1983) Indole-3-acetic acid and IAA conjugates applied to bean stem sections. Plant Physiol 73:130–134

    PubMed  CAS  Google Scholar 

  • Cohen JD (1982) Identification and quantitative analysis of indole-3-acetyl-l-aspartate from seeds of Glycine max L. Plant Physiol 70:749–753

    PubMed  CAS  Google Scholar 

  • Cohen JD, Bandurski RS (1982) Chemistry and physiology of the bound auxins. Annu Rev Plant Physiol 33:403–430

    Article  CAS  Google Scholar 

  • Duddeck H, Hiegemann M, Simeonov MF, Kojić-Prodić B, Nigović B, Magnus V (1989) Conformational study of some amino acid conjugates of indol-3-ylacetic acid (IAA) by 1H-NOE-difference spectroscopy. Structure/auxin activity relationships. Z Naturforsch 44c:543–554

    Google Scholar 

  • Ehmann A (1977) The van Urk-Salkowski reagent—a sensitive and specific reagent for silica gel thin-layer chromatographic detection and identification of indole derivatives. J Chromatogr 132:267–276

    Article  PubMed  CAS  Google Scholar 

  • Epstein E, Baldi BG, Cohen JD (1986) Identification of indole-3-acetyl-glutamate from seeds of Glycine max L. Plant Physiol 80:256–258

    PubMed  CAS  Google Scholar 

  • Feung CS, Hamilton RH, Mumma RO (1975) Indole-3-acetic acid. Mass spectra and chromatographic properties of amino acid conjugates. J Agric Food Chem 23:1120–1124

    Article  CAS  Google Scholar 

  • Good NE (1956) The synthesis of indole-3-acetyl-d, l-aspartic acid and related compounds. Can J Chem 34:1356–1358

    Article  CAS  Google Scholar 

  • Hangarter RP, Peterson MD, Good NE (1980) Biological activities of indoleacetyl amino acids and their use as auxins in tissue culture. Plant Physiol 65:761–767

    PubMed  CAS  Google Scholar 

  • Hangarter RP, Good NE (1981) Evidence that IAA conjugates are slow-release sources of free IAA in plant tissues. Plant Physiol 68:1424–1427

    PubMed  CAS  Google Scholar 

  • Hutzinger O, Kosuge T (1968) Microbial synthesis and degradation of indole-3-acetic acid. III. The isolation and characterization of indole-3-acetyl-l-lysine. Biochemistry 7:601–605

    Article  PubMed  CAS  Google Scholar 

  • Kojić-Prodić B, Nigović B, Horvatić D, Ružić-Toroš Ž, Magnus V (1990) Structural studies on conjugates of the plant growth hormone (auxin) with amino acids. XVth Congress of the International Union of Crystallography (Bordeaux, France, July 19–28): Collected Abstracts C-161

  • Kojić-Prodić B, Nigović B, Horvatić D, Ružić-Toroš Ž, Magnus V, Duax WL, Stezowski JJ, Bresciani-Pahor N (1991) Comparison of the structures of the plant growth hormone indole-3-acetic acid, and six of its amino-acid conjugates. Acta Cryst B47:107–115

    Google Scholar 

  • Magnus V, Nigović B, Hangarter RP, Good NE (1992) tN-(Indol-3-ylacetyl) amino acids as sources of auxin in plant tissue culture. J Plant Growth Regul (in press)

  • Mollan RC, Donelly DMX, Harmey MA (1972) Synthesis of indole-3-acetyl aspartic acid. Phytochemistry 11:1485–1488

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Plüss R (1987) Rhizogenese in Grünstecklingen von Populus tremula: Wirkung, Translokation und Metabolismus von exogen applizierter Indolessigsäure. Ph D Thesis, University of Freiburg, Switzerland

    Google Scholar 

  • Tsurumi S, Wada S (1986) Dioxindole-3-acetic acid conjugates formation from indole-3-acetyl aspartic acid in Vicia seedlings. Plant Cell Physiol 27:1513–1522

    CAS  Google Scholar 

  • Wieland T, Hörlein G (1955) Synthesen einiger β-Indolacetylaminosäuren und -peptide. Justus Liebigs Ann Chem 591:192–199

    Article  CAS  Google Scholar 

  • Wodzicki TJ, Pharis RP, Wodzicki AB (1987) Interaction of indoleacetic acid with its inositol and glucoside conjugates in Avena coleoptile curvature. Plant Physiol 84:1139–1142

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Rudjer Bošković Institute, P.O. Box 1016, HR-41001, Zagreb, Croatia

    Volker Magnus

  2. Department of Plant Biology, Ohio State University, 1735 Neil Avenue, 43210-1293, Columbus, Ohio, USA

    Roger P. Hangarter

  3. Department of Botany and Plant Pathology, Michigan State University, 48824-1312, East Lansing, Michigan, USA

    Norman E. Good

Authors
  1. Volker Magnus
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Roger P. Hangarter
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Norman E. Good
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Magnus, V., Hangarter, R.P. & Good, N.E. Interaction of free indole-3-acetic acid and its amino acid conjugates in tomato hypocotyl cultures. J Plant Growth Regul 11, 67–75 (1992). https://doi.org/10.1007/BF00198017

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00198017

Keywords

Search

Navigation