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Determination of domoic acid by two different versions of a competitive enzyme-linked immunosorbent assay (ELISA)

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  1. Chevrier D, Rasmussen SR, Guesdon J-L (1993) PCR product quantification by non-radioactive hybridization procedures using an oligonucleotide covalently bound to microwells. Mol Cell Probes 7:187–197

    Google Scholar 

  2. Iverson F, Truelove J, Nera E, Tryphonas L, Campbell J, Lok E (1989) Domoic acid poisoning and mussel-associated intoxication: preliminary investigations into the response of mice and rats to toxic mussel extract. Food Chem Toxicol 27:377–384

    Google Scholar 

  3. Lawrence JF, Charbonneau CF, Menard C, Quilliam MA, Sim PG (1989) Liquid chromatographic determination of domoic acid in shellfish products using the AOAC paralytic shellfish poison extraction procedure. J Chromatogr 462:349–356

    Google Scholar 

  4. Newsome H, Truelove J, Hierlihy L, Collins P (1991) Determination of domoic acid in serum and urine by immunochemical analysis. Bull Environ Contam Toxicol 47:329–334

    Google Scholar 

  5. Pocklington R, Milley JE, Gates SS, Bird CJ, DeFreitas ASW, Quilliam MA (1990) Trace determination of domoic acid in seawater and phytoplankton by high performance liquid chromatography of the fluorenylmethoxycarbonyl (FMOC) derivative. Int J Environ Anal Chem 38:351–368

    Google Scholar 

  6. Quilliam MA, Sim PG, McCulloch, McInnes AG (1989) High performance liquid chromatography of domoic acid, a marine neurotoxin, with application of shellfish and plankton. Int J Environ Anal Chem 36:139–154

    Google Scholar 

  7. Rasmussen SE (1990) Covalent immobilization of biomolecules onto polystyrene microwells for use in biospecific assay. Ann Biol. Clin 48:647–650

    Google Scholar 

  8. Rasmussen SR, Larsen MR, Rasmussen SE (1991) Covalent immobilization of DNA onto polystyrene microwells: the molecules are only bound at the 5′ end. Anal Biochem 198:138–142

    Google Scholar 

  9. Søndergård-Andersen J, Lauritzen E, Lind K, Holm A (1990) Covalently linked peptides for enzyme-linked immunosorbent assay. J Immunol Methods 131:99–104

    Google Scholar 

  10. Subba Rao DV, Quilliam MA, Pocklington R (1988) Domoic acid-a neurotoxic amino acid produced by the marine diatom Nitzschia pungens in culture. Can J Fisheries Aquat Sci 45:2076–2079

    Google Scholar 

  11. Todd ECD (1993) Domoic acid and amnesic shellfish poisoning — A review. J. Food Prot. 56:69–83

    Google Scholar 

  12. Wright JLC, Boyd RK, De Freitas ASW, Falk M, Foxall RA, Jamieson WD, Laycock MV, McCulloch AW, McInnes AG, Odense P, Pathak VP, Quilliam MA, Ragan MA, Sim PG, Thibault P, Walter JA, Gilgan M, Richard DJA, Dwar D (1989) Identification of domoic acid, a neuroexitatory amino acid, in toxic mussels from eastern Prince Edward Island. Can J Chem 67:481–490

    Google Scholar 

  13. Yonezawa S, Kambegawa A, Tokudome S (1993) Covalent coupling of a steroid to microwell plates for use in a competitive enzyme-linked immunosorbent assay. J Immunol Methods 166:55–61

    Google Scholar 

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Correspondence to J. E. Stewart.

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Osada, M., Marks, L.J. & Stewart, J.E. Determination of domoic acid by two different versions of a competitive enzyme-linked immunosorbent assay (ELISA). Bull. Environ. Contam. Toxicol. 54, 797–804 (1995).

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  • Waste Water
  • Water Management
  • Water Pollution
  • Immunosorbent Assay
  • Domoic Acid