Abstract
Citrate uptake into barley (Hordeum vulgare L.) mesophyll vacuoles was found to be saturable with a K m of about 200 μM. Uptake appears to occur via the citrate3- form, as indicated by concentration-dependent uptake studies at different pHs. Free citrate and not the Mg-citrate complex was taken up by the vacuoles, even though slow transport of the Mg complex could not be excluded. Citrate transport into vacuoles was competitively inhibited by malate (K i=0.68 mM). Various organic acids and protein-modifying agents affected the uptake of malate and citrate to a similar extent. These results indicate that both organic acids cross the tonoplast by means of the same carrier. Accumulation of citrate was ATP-dependent and could be inhibited by ionophores. Bovine serum albumin strongly stimulated citrate uptake, but other proteins tested did not show a similar stimulatory effect.
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Abbreviations
- BSA:
-
bovine serum albumin
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We wish to thank Esther Vogt for her help with the experiments and Professor N. Amrhein (ETH, Zürich, Switzerland) and Dr. Michael Kertesz (ETH, Zürich) for helpful discussions. This work was supported by the Swiss National Foundation grant No. 31-25196.88.
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Rentsch, D., Martinoia, E. Citrate transport into barley mesophyll vacuoles — comparison with malate-uptake activity. Planta 184, 532–537 (1991). https://doi.org/10.1007/BF00197903
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DOI: https://doi.org/10.1007/BF00197903