Abstract
An electron-microscopic cytochemical method was used to localize diamine oxidase (DAO) in pea and polyamine oxidase (PAO) in maize (Zea mays L.). The method, based on the precipitation of amine-oxidase-generated H2O2 by CeCl3, was shown to be specific for DAO and PAO and permitted their localization in plant tissues with a high degree of resolution. Both enzymes are localized exclusively in the cell wall. Both DAO- and PAO-activity staining is most intense in the middle lamellar region of the wall and in cells exhibiting highly lignified walls. The oxidases could provide H2O2 for peroxidase-mediated cross-linking reactions in the cell wall and may, in this capacity, play a role in the regulation of plant growth.
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Abbreviations
- AG:
-
1-aminoguanidine
- AT:
-
3-amino-1,2,4-triazole
- β-HEH:
-
β-hydroxyethylhydrazine
- DAO(s):
-
diamine oxidase(s)
- PAO(s):
-
polyamine oxidase(s)
- Put:
-
putrescine
- Spd:
-
spermidine
- Spm:
-
spermine
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The authors wish to thank Nancy Piatczyc for the technical assistance with electron-microscopy studies. We are grateful to Dr. Stanley J. Roux, University of Texas at Austin, for providing us with samples of maize cell-wall exudates. This work was supported by grants to R.D.S from the National Aeronautics and Space Administration (NAGW-1049 and NAGW-1382).
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Slocum, R.D., Furey, M.J. Electron-microscopic cytochemical localization of diamine and polyamine oxidases in pea and maize tissues. Planta 183, 443–450 (1991). https://doi.org/10.1007/BF00197744
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DOI: https://doi.org/10.1007/BF00197744