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Diurnal periodicity of chalcone-synthase activity during the development of oat primary leaves

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Abstract

Chalcone-synthase (CHS) activity was followed during the development of primary leaves of oat (Avena sativa L.) seedlings grown under different illumination conditions. Continuous darkness and continuous light resulted in similar time courses of enzyme activity. The maximum of CHS activity in etiolated leaves was delayed by 1 d and reached about half the level of that of light-grown leaves. In seedlings grown under defined light-dark cycles a diurnal rhythm of CHS activity and its protein level was observed which followed the rhythm of CHS-mRNA translational activity (Knogge et al. 1986). This rhythm persisted in continuous light after a short-term pre-exposure to the light-dark cycle but not in continuous darkness.

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Abbreviations

CHS:

chalcone synthase

PAL:

phenylalanine ammonio lyase

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Authors and Affiliations

  1. Botanisches Institut der Universität Köln, Gyrhofstrasse 15, W-5000, Köln, 41, Germany

    Hans-Jürgen Peter & Gottfried Weissenböck

  2. Botanisches Institut der Universität Bonn, Kirschallee 1, W-5300, Bonn 1

    Christiane Krüger-Alef & Klaus Brinkmann

  3. Abteilung Biochemie, Max Planck Institut für Züchtungsforschung, Egelspfad, W-5000, Köln, 30, Germany

    Wolfgang Knogge

Authors
  1. Hans-Jürgen Peter
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  2. Christiane Krüger-Alef
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  3. Wolfgang Knogge
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  4. Klaus Brinkmann
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  5. Gottfried Weissenböck
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Additional information

Financial support by the Deutsche Forschungsgemeinschaft is gratefully acknowledged (G.W., We 630/9-7; We 630/10-1). Thanks are given to Dr. St. Kellam (Department of Plant Microbiological Sciences, University of Canterbury, New Zealand) for correcting the English.

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Peter, HJ., Krüger-Alef, C., Knogge, W. et al. Diurnal periodicity of chalcone-synthase activity during the development of oat primary leaves. Planta 183, 409–415 (1991). https://doi.org/10.1007/BF00197740

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  • DOI: https://doi.org/10.1007/BF00197740

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