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Transport of inorganic phosphate and C3- and C6-sugar phosphates across the envelope membranes of potato tuber amyloplasts

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Abstract

Amyloplasts have been isolated from tubers of potato plants (Solarium tuberosum. cv. Desirée). As it is difficult to isolate amyloplasts that have a high starch content, we used transformed plants in which the content of starch was reduced. This was achieved by decreasing the activity of ADP-glucose pyrophosphorylase by antisense techniques (Müller-Röber et al., 1992, EMBO. 11, 1229–1238). In the isolated plastids the activity of glutamine-oxoglutarate-aminotransferase (glutamate synthase, EC 2.6.1.53) was dependent upon the intactness of the plastids. For the supply of redox equivalents the addition of glucose-6-phosphate (Glc6P) was required. Glucose-1-phosphate (Glc1P) did not support glutamate synthesis. Plastids were treated with Triton X-100 and the solubilized proteins reconstituted into liposomes. Transport measurements with these liposomes revealed that inorganic phosphate (Pi), dihydroxyacetone phosphate (DHAP), 3-phosphoglycerate and Glc6P are transported in a counter-exchange mode. Transport of phosphoenolpyruvate was low and Glc1P was virtually not transported in exchange for Pi. Kinetic constants were determined for the Pi/Pi and Glc6P/Pi counter exchanges. For comparison, proteins of mitochondria from potato tubers and pea leaves were reconstituted into liposomes. As expected, the Pi/Pi exchange across the mitochondrial membrane was not affected by DHAP and Glc6P. Kinetic constants of the Pi/Pi counter exchange were determined for potato tuber mitochondria.

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Abbreviations

DHAP:

dihydroxyacetone phosphate

Glc1P:

glucose-1-phosphate

Glc6P:

glucose-6-phosphate

PEP:

Phosphoenolpyruvate

3-PGA:

3-phosphoglycerate

Pi:

inorganic phosphate

Tricine:

N-[2-hydroxy-1,1-bis(hydroxymethyl)-ethyl] glycine

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Authors and Affiliations

  1. Institut für Biochemie der Pflanze, Universität Göttingen, Untere Karspüle 2, D-37073, Göttingen, Germany

    Karin Schott, Sieglinde Borchert, Bernd Müller-Röber & Hans Walter Heldt

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  1. Karin Schott
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  2. Sieglinde Borchert
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  3. Bernd Müller-Röber
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  4. Hans Walter Heldt
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This work was supported by Deutsche Forschungsgemeinschaft.

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Schott, K., Borchert, S., Müller-Röber, B. et al. Transport of inorganic phosphate and C3- and C6-sugar phosphates across the envelope membranes of potato tuber amyloplasts. Planta 196, 647–652 (1995). https://doi.org/10.1007/BF00197327

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  • DOI: https://doi.org/10.1007/BF00197327

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