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Extracellular deamination of l-amino acids by Chlamydomonas reinhardtii cells

Planta volume 182, pages 194–198 (1990)Cite this article

Abstract

When grown in the light and in a Tris-acetate phosphate medium, cells of Chlamydomonas reinhardtii Dang. can use the following l-amino acids as a sole nitrogen source: asparagine, glutamine, arginine, lysine, alanine, valine, leucine, isoleucine, serine, methionine, histidine, and phenylalanine, whereas, in the absence of acetate, the cells only used l-arginine. The utilization system in the acetate medium consisted of an extracellular deaminating activity induced by l-amino acids; it took between 10 to 30 h before the system appeared in cells previously grown with ammonium. This deaminase activity was nonspecific, required an organic carbon source for its de-novo synthesis, and was sensitive to high ammonium concentration and light deprivation.

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Abbreviations

HPLC:

high-performance liquid chromatography

TAP:

Tris-acetate-phosphate

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Affiliations

  1. Departamento de Bioquímica y Biología Molecular y Fisiología, Facultad de Ciencias, Universidad de Córdoba, Avda. S. Alberto Magno, s/n, E-14071, Córdoba, Spain

    J. Muñoz-Blanco, J. Hidalgo-Martínez & J. Cárdenas

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  1. J. Muñoz-Blanco
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  2. J. Hidalgo-Martínez
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  3. J. Cárdenas
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Additional information

This work was supported by a grant of the CAICYT, Spain. The secretarial assistance of C. Santos and I. Molina is gratefully acknowledged.

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Muñoz-Blanco, J., Hidalgo-Martínez, J. & Cárdenas, J. Extracellular deamination of l-amino acids by Chlamydomonas reinhardtii cells. Planta 182, 194–198 (1990). https://doi.org/10.1007/BF00197110

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  • DOI: https://doi.org/10.1007/BF00197110

Key words

  • L-amino acids (utilization)
  • Chlamydomonas
  • Deaminase (extracellular)