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Purification and properties of a glyphosate-tolerant 5-enolpyruvylshikimate 3-phosphate synthase from the cyanobacterium Anabaena variabilis

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Abstract

5-Enolpyruvylshikimate 3-phosphate (EPSP) synthase (3-phosphoshikimate 1-carboxyvinyltransferase; EC 2.5.1.9) from the glyphosate-tolerant cyanobacterium Anabaena variabilis (ATCC 29413) was purified to homogeneity. The enzyme had a similar relative molecular mass to other EPSP synthases and showed similar kinetic properties except for a greatly elevated K i for the herbicide glyphosate (approximately ten times higher than that of enzymes from other sources). With whole cells, the monoisopropylamine salt of glyphosate was more toxic than the free acid but the effects of the free acid and monoisopropylamine salt on purified EPSP synthase were identical.

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Abbreviations

EPSP:

5-enolpyruvylshikimate 3-phosphate

Mr :

relative molecular mass

PEP:

phosphoenolpyruvate

SDS-PAGE:

sodium dodecyl sulphate-polyacrylamide gel electrophoresis

S3P:

shikimate 3-phosphate

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Authors and Affiliations

  1. AFRC Research Group on Cyanobacteria and Department of Biological Sciences, University of Dundee, DD1 4HN, Dundee

    Hilary A. Powell, Nigel W. Kerby & Peter Rowell

  2. Department of Biochemistry, University of Glasgow, G12 8QQ, Glasgow, UK

    David M. Mousdale & John R. Coggins

Authors
  1. Hilary A. Powell
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  2. Nigel W. Kerby
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  3. Peter Rowell
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  4. David M. Mousdale
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  5. John R. Coggins
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The funding of this work by the Agricultural and Food Research Council and the University of Dundee Research Initiatives Programme is gratefully acknowledged.

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Powell, H.A., Kerby, N.W., Rowell, P. et al. Purification and properties of a glyphosate-tolerant 5-enolpyruvylshikimate 3-phosphate synthase from the cyanobacterium Anabaena variabilis . Planta 188, 484–490 (1992). https://doi.org/10.1007/BF00197039

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