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Germination-specific lipid transfer protein cDNAs in Brassica napus L.

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Abstract

Three cDNA clones encoding lipid transfer proteins (LTPs) were isolated by applying the rapid amplification of cDNA ends (RACE) protocol to imbibed seeds and germinating seedlings of Brassica napus. The deduced amino-acid sequences show a great degree of homology and they exhibit the common features shared by all LTPs. Their expression pattern indicates a strong developmental, hormonal, and environmental regulation. They are expressed only in cotyledons and hypocotyls of germinating seedlings and their levels of expression increase upon treatment with cis-abscisic acid and NaCl. Their distribution in the cotyledons of young seedlings is suggestive of a role related to the mobilization of lipid reserves.

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Abbreviations

ABA:

cis-abscisic acid

LTP:

lipid transfer protein

RACE-PCR:

rapid amplification of cDNA ends-polymerase chain reaction

References

  • Aguirre PJ, Smith AG (1993) Molecular characterization of a gene encoding a cysteine-rich protein preferentially expressed in anthers of Lycopersicon esculentum. Plant Mol Biol 23: 477–487

    Google Scholar 

  • Arondel V, Kader J-C (1990) Lipid transfer in plants. Experientia 46: 579–585

    Google Scholar 

  • Bernhard WR, Thoma S, Botella J, Somerville CR (1991) Isolation of a cDNA clone for spinach lipid transfer protein and evidence that the protein is synthesized by the secretory pathway. Plant Physiol 95: 164–170

    Google Scholar 

  • Fleming AJ, Mandel T, Hofmann S, Sterk P, de Vries SC, Kuhlemeier C (1992) Expression pattern of a tobacco lipid transfer protein gene within the shoot apex. Plant J 2: 855–862

    Google Scholar 

  • Foster GD, Robinson SW, Blundell RP, Roberts MR, Hodge R, Draper J, Scott RJ (1992) A Brassica napus mRNA encoding a protein homologous to phospholipid transfer proteins, is expressed specifically in the tapetum and developing microspores. Plant Sci 84: 187–192

    Article  CAS  Google Scholar 

  • Fourney RM, Miyakoshi J, Day III RS, Paterson M (1988) Northern blotting: Efficient RNA staining and transfer. Focus 10: 5–7

    Google Scholar 

  • Frohman MA, Duch MK, Martin GR (1988) Rapid production of full-length cDNAs from rare transcritps: Amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci USA 85: 8998–9002

    CAS  PubMed  Google Scholar 

  • Gausing K (1994) Lipid transfer protein genes specifically expressed in barley leaves and coleoptiles. Planta 192: 574–580

    Google Scholar 

  • Grosbois M, Guerbette F, Kader J-C (1989) Changes in level and activity of phospholipid transfer protein during maturation and germination of maize seeds. Plant Physiol 90: 1560–1564

    Google Scholar 

  • Grosbois M, Guerbette F, Jolliot A, Quintin F, Kader J-C (1993) Control of maize lipid transfer protein activity by oxido-reducing conditions. Biochim Biophys Acta 1170: 197–203

    Google Scholar 

  • Madrid SM (1991) The barley lipid transfer protein is targeted into the lumen of the endoplasmic reticulum. Plant Physiol Biochem 29: 695–703

    Google Scholar 

  • Madrid SM, von Wettstein D (1991) Reconciling contradictory notions on lipid transfer proteins in higher plants. Plant Physiol Biochem 29: 05–711

    Google Scholar 

  • Munro S, Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48: 899–907

    Google Scholar 

  • Murray EE, Lotzer J, Eberle M (1989) Codon usage in plants. Nucleic Acids Res 17: 477–498

    Google Scholar 

  • Ortigao RJF, Jirikowski GF, Seliger H (1989) 5-Bromouridinilated oligonucleotide for hybridization analysis of DNA and RNA on membranes and in situ. Nucleosides nucleotides 8: 805–813

    Google Scholar 

  • Ostergaard J, Vergnolle C, Schoentgen F, Kader J-C (1993) Acyl-binding/lipid transfer protein from rape seedlings, a novel category of proteins interacting with lipids. Biochim Biophys Acta 1170: 109–117

    Google Scholar 

  • Pyee J, Kolattukudy PE (1995) The gene for the major cuticular wax-associated protein and three homologous genes from broccoli (Brassica oleracea) and their expression patterns. Plant J 7: 49–59

    Google Scholar 

  • Pyee J, Yu H, Kolattukudy PE (1994) Identification of a lipid transfer protein as the major protein in the surface wax of broccoli (Brassica oleracea) leaves. Arch Biochem Biophys 331: 460–468

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY

    Google Scholar 

  • Sossountzov L, Maldiney R, Sotta B, Sabbagh I, Habricot Y, Bonnet M, Miginiac E (1988) Immunocytochemical localisation of cytokinins in graigella tomato and a sideshootless mutant. Planta 175: 291–304

    Google Scholar 

  • Sterk P, Booij H, Schellekens GA, van Kamen A, de Vries SC (1991) Cell specific expression of the carrot EP2 lipid transfer protein gene. Plant Cell 3: 907–921

    Google Scholar 

  • Takishima K, Watanabe S, Yamada M, Suga T, Mamiya G (1988) Amino acid sequences of two nonspecific lipid-transfer proteins from germinated castor bean. Eur J Biochem 177: 241–249

    Google Scholar 

  • Terras FRG, Goderis IJ, van Leuven F, Vanderleyden J, Cammue BPA, Broekaert WF (1992) In vitro antifungal activity of a radish (Raphanus sativu L.) seed protein homologous to nonspecific lipid transfer proteins. Plant Physiol 100: 1055–1058

    Google Scholar 

  • Thoma S, Hecht U, Kippers A, Botella J, De Vries S, Somerville C (1994) Tissue-specific expression of a gene encoding a cell wall-localized lipid transfer protein from Arabidopsis. Plant Physiol 105: 35–45

    Google Scholar 

  • Torres-Schumann S, Godoy JA, Pnintor-Toro JA (1992) A probable lipid transfer protein gene is induced by NaCl in stems of tomato plants. Plant Mol Biol 18: 749–757

    Google Scholar 

  • Tsuboi S, Suga T, Takishima K, Mamiya G, Matsui K, Ozeki Y, Yamada M (1991) Organ-specific occurrence and expression of the isoforms of nonspecific lipid transfer protein In castor bean seedlings, and molecular cloning of a full-length cDNA for a cotyledon-specific isoform. J Biochem 110: 823–831

    Google Scholar 

  • Tsuboi S, Osafune T, Tsugeki R, Nishimura M, Yamada M (1992) Nonspecific lipid transfer protein in castor bean cotyledon cells: subcellular localization and a possible role in lipid metabolism. J Biochem 111: 500–508

    Google Scholar 

  • Verwoerd TC, Dekker BMM, Hoekema A (1989) A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Res 17: 2362

    CAS  PubMed  Google Scholar 

  • von Heijne G (1985) A new method for predicting signal sequence cleavage sites. Nucleic Acids Res 14: 4683–4690

    Google Scholar 

  • Walbot V (1988) Preparation of DNA from single rice seedlings. Rice Genet Newslett 5: 149–151

    Google Scholar 

  • Weig A, Komor E (1992) The lipid transfer protein C of Ricinus communis L.: Isolation of two cDNA sequences which are strongly and exclusively expressed in cotyledons after germination. Planta 187: 367–371

    Google Scholar 

  • Zeff RA, Geliebter J (1987) Oligonucleotide probes for genomic DNA blots. Focus 9: 1–3

    Google Scholar 

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Dr. V. Arondel (University Paris VI, Paris, France) is gratefully acknowledged for helpful discussions and for critically reading the manuscript. F. Guerbette (University Paris VI, Paris, France) is acknowledged for communicating unpublished data. Dr. B. Sotta (University Paris VI, Paris, France) is acknowledged for helpful discussions and guidance concerning the immunolocalization and Y. Habricot (University Paris VI, Paris, France) for excellent technical assitance. I. A. Soufleri was supported by a BRIDGE grant through the European Postgraduate Training Network; Application of Genetic Engineering and Molecular Biology for Seed Improvement.

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Soufleri, I.A., Vergnolle, C., Miginiac, E. et al. Germination-specific lipid transfer protein cDNAs in Brassica napus L.. Planta 199, 229–237 (1996). https://doi.org/10.1007/BF00196563

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  • DOI: https://doi.org/10.1007/BF00196563

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