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A statistical analysis of some genetical, physiological and anatomical parameters of the development of in situ- and in vitro-grown ovules from intra- and interspecific crosses in the genus Beta

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Summary

Structural observations on in situ- and in vitro-grown ovules from different intra- and interspecific crosses in the genus Beta were tested using computer registration and statistical analysis. In intra- as well as interspecific crosses embryo development occurs with the same rate independent of growth conditions. Therefore, with respect to growth rate, embryo development seems to be highly autonomous, provided that nutrient influx into the ovule is sufficient. The endosperm develops significantly more rapidly during in vitro culture than in situ. A large degree of variability with respect to structural changes in the ovule tissue during in vitro culture is observed in and between the crosses. In general, the interspecific cross responds more rapidly on in vitro culture. In all crosses the embryo, although genetically identical to the suspensor, shows a higher degree of response to in vitro culture than the embryo itself. Early suspensor degeneration in the interspecific cross is the only observed difference between the crosses, which could explains the lack of root formation in seedlings of interspecific hybrids. The use of statistical analysis on the anatomical parameters used to compare treatment as well as crosses has proven to be an efficient and novel approach to plant reproduction biology.

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  1. University of Copenhagen, Botanical Laboratory, Gothersgade 140, DK-1123, Copenhagen K, Denmark

    Lone Brunn

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Brunn, L. A statistical analysis of some genetical, physiological and anatomical parameters of the development of in situ- and in vitro-grown ovules from intra- and interspecific crosses in the genus Beta . Sexual Plant Reprod 4, 118–125 (1991). https://doi.org/10.1007/BF00196498

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