Abstract
Paclobutrazol (PBZ), a triazole growth retardant known to improve tolerance of various species to stress, was incorporated into the in vitro rooting medium of Prunus serotina var. virens at rates of 0.00, 0.15, 0.30, and 0.60 mg/L with and without 1.0 mg/L indolebutyric acid (IBA). PBZ significantly reduced shoot growth in vitro but increased/improved the quality and coloration. The percentage of water loss from detached leaves of in vitro plantlets was significantly reduced by PBZ and IBA. At 4 weeks after transfer to the greenhouse, survival was significantly improved by PBZ, IBA, and the combination. Incorporation of PBZ in vitro better enables Prunus serotina plantlets to withstand the stresses associated with acclimatization.
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References
Anderson C, Meagher GW (1978) Cost of propagating broccoli plants through tissue culture. HortScience 12:543–544
Brainerd KE, Fuchigami LH (1981) Acclimatization of aseptically cultured apple plants to low relative humidity. J Amer Soc Hort Sci 106:515–518
Brainerd KE, Fuchigami LH, Kwartkowski S, Clark CS (1981) Leaf anatomy and water stress of aseptically cultured “Pixy” plum grown under different environments. Hort-Science 16:173–175
Crane J, Hughes H (1990) Medium overlays for improved hardening of micropropagated potatoes. HortScience 25:794–795
Davis TD (1987) Interior performance of three foliage plant species treated with Paclobutrazol. Appl Agr Res 2:120–123
Davis TD, Steffens GL, Sankhla N (1988) Triazole plant growth regulators. Hort Rev 10:63–105
Donnelly DJ (1990) The culture-induced phenotype. Proc Plant Growth Reg Soc Am 17:47–60
Fujiwara K, Kozai T, Watanabe I (1988) Development of a photoautotrophic tissue culture system for shoots and/or plantlets at rooting and acclimatization stages. Acta Hort 230:129–135
Kavanaugh KL (1987) Acclimatization of Prunus serotina Ehrh. Propagation in vitro. MS Thesis. SUNY College of Environmental Science and Forestry, Syracuse, NY
Khunachak A, Chin DK, Gianfagna TL, Gianfagna T (1987) Promotions of asparagus shoot and root growth by growth retardants. Plant Cell Tissue Organ Culture 11:97–110
McVaugh R (1951) A revision of the North American black cherries (Prunus serotina Ehrh. and relatives). Brittonia 7(4): 279–315
Marino G (1986) The effect of Paclobutrazol on the in vitro rooting of a Prunus species hybrid rootstock. Riv Ortoflorofrutt It 70:267–275
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Orzolek MD (1987) Use of growth retardants for tomato transplant production. HortScience 22:1161 (Abstr)
Orzolek MD (1989) Growth retardants improve plant performance. Am Veg Grow 37:42–44
Sankhla A, Davis TD, Sankhla D, Sankhla N, Upadhyaya A, Joshi S (1991) Triazole plant growth regulators influence somatic embryogenesis and plantlet regeneration in tissue cultures of Echinochloa frumentacea. Proc Plant Growth Reg Soc Am 18:84–89
Smith EF, Roberts AV, Mottley J (1990) The preparation in vitro of chrysanthemum for transplantation to soil 2. Improved resistance to desiccation conferred by Paclobutrazol. Plant Cell Tissue Organ Culture 21:133–140
Smith EF, Gribaudo I, Roberts AV, Mottley J (1992) Paclobutrazol and reduced humidity improve resistance to wilting of micropropagated grapevine. HortScience 27:111–113
Snir I (1988) Influence of Paclobutrazol on in vitro growth of sweet cherry shoots. HortScience 23:304–305
Steffens GL, Wang SY (1986) Biochemical and physiological alterations in apple trees caused by a gibberellin biosynthesis inhibitor, Paclobutrazol. Acta Hort 179:433–442
Tricoli DM, Maynard CA, Drew AP (1985) Tissue culture propagation of mature trees of Prunus serotina Ehrh. I. Establishment, multiplication and rooting in vitro. Forest Science 31:201–208
Wample RL, Culver EV (1983) The influence of Paclobutrazol, a new growth regulator, on sunflowers. J Amer Soc Hort Sci 108:122–125
Wardle K, Dobbs EB, Short KC (1983) In vitro acclimatization of aseptically cultured plantlets to humidity. J Amer Soc Hort Sci 108:386–389
Ziv M, Meir G, Malevy AM (1983) Factors influencing the production of hardened glaucous carnation plantlets in vitro. Plant Cell Tissue Organ Culture 2:55–65
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Eliasson, M.K., Beyl, C.A. & Barker, P.A. In vitro responses and acclimatization of Prunus serotina with paclobutrazol. J Plant Growth Regul 13, 137–142 (1994). https://doi.org/10.1007/BF00196377
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DOI: https://doi.org/10.1007/BF00196377