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Auxin-induced H+-pump stimulation does not depend on the presence of epidermal cells in corn coleoptiles

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Abstract

Cell-wall acidification and electrical reactions (depolarization and hyperpolarization) are typical auxin responses in maize (Zea mays L.) coleoptiles. In an attempt to test the role of the outer epidermis in these responses, they have been measured and compared in intact and peeled coleoptile fragments. To exclude interactions between parenchymal and epidermal cells, the coleoptile pieces were completely stripped of their outer epidermis. This preparation was monitored by means of a scanning electron microscope. When externally applied indole-3-acetic acid was tested, we found that neither cell-wall acidification nor the electrical membrane responses depended on the presence of intact epidermal cells.

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Abbreviations

IAA:

Indole-3-acetic acid

MES:

2-[N morpholino-ethane-sulfonic acid

TRIS:

2-Amino-2-hydroxymethyl-1,3-propanediol

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Authors and Affiliations

  1. Institut für allgemeine Botanik und Pflanzenphysiologie, Senckenbergstrasse 17-21, W-6300, Giessen, Federal Republic of Germany

    Winfried Stefan Peters, Ursula Richter & Hubert H. Felle

Authors
  1. Winfried Stefan Peters
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  2. Ursula Richter
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  3. Hubert H. Felle
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Additional information

We thank Kuki Kaethner for her excellent technical assistance. This work was supported by the Hessische Graduiertenförderung and the Deutsche Forschungsgemeinschaft.

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Peters, W.S., Richter, U. & Felle, H.H. Auxin-induced H+-pump stimulation does not depend on the presence of epidermal cells in corn coleoptiles. Planta 186, 313–316 (1992). https://doi.org/10.1007/BF00196261

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  • DOI: https://doi.org/10.1007/BF00196261

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