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Differential accumulation of the 10-, 16- and 23-kDa peripheral components of the water-splitting complex of photosystem II in mesophyll and bundle-sheath chloroplasts of the dicotyledonous C4 plant Flaveria trinervia (Spreng.) C. Mohr

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Abstract

The differential expression of PSII genes was investigated in mesophyll and bundle-sheath cells of Flaveria trinervia, a dicotyledonous C4 plant of the NADP-malic enzyme type. A comprehensive immunoblot analysis showed that three extrinsic proteins of the water-splitting complex (10, 16 and 23 kDa) are selectively depleted in mature bundle-sheath chloroplasts. In contrast, the reaction-centre core remained virtually unaffected as inferred from the abundance of the 47-kDa chlorophyll-a-binding protein, the D1 and D2 polypeptides, cytochrome b559 and the 34-kDa polypeptide. The selective depletion of the 10-, 16- and 23-kDa polypeptides in bundle-sheath chloroplasts was paralleled by a diminished PSII capacity. On the basis of oxygen evolution in the presence of the artifical electron acceptor 2,5-dimethyl-p-benzoquinone, bundle-sheath chloroplasts maintained up to 23 % of the PSII capacity shown by mesophyll chloroplasts. However, the levels of the 10-, 16- and 23-kDa proteins and, concomitantly, PSII activity varied to some degree and appeared to be correlated with environmental factors caused by seasonal changes. The selective depletion of the three members of the water-splitting complex was not reflected at the transcript level. The corresponding mRNAs were detectable in considerable amounts in bundle-sheath cells, indicating that the depletion of these proteins is regulated by post-transcriptional events. These findings reinforce the view that the peripheral proteins of the water-splitting complex are a focal point for controlling PSII activity in bundle-sheath chloroplasts of both mono- and dicotyledonous C4 plants of the NADP-malic enzyme subtype.

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Abbreviations

DMBQ:

2,5-dimethyl-p-benzoquinone

PEPCase:

phosphoenolpyruvate carboxylase

Chl:

chlorophyll

SDS:

sodium dodecyl sulfate

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Authors and Affiliations

  1. Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine Universität Düsseldorf, W-4000, Düsseldorf 1, Federal Republic of Germany

    Michael U. Höfer, Uwe J. Santore & Peter Westhoff

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  1. Michael U. Höfer
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  2. Uwe J. Santore
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  3. Peter Westhoff
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Additional information

We thank the staff of the greenhouse under the direction of Mr. W. Rogmann for the supply of excellent plant material, M. Streubel for her photographic work and W. Poetsch and J. Hermans for providing the PEPCase cDNA clone. M.H. thanks Professor G.H. Krause and B. Weyers (Institute of Plant Biochemistry, University of Düsseldorf) for introducing him into the polarographic techniques. This work was supported by Deutsche Forschungsgemeinschaft (SFB 189).

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Höfer, M.U., Santore, U.J. & Westhoff, P. Differential accumulation of the 10-, 16- and 23-kDa peripheral components of the water-splitting complex of photosystem II in mesophyll and bundle-sheath chloroplasts of the dicotyledonous C4 plant Flaveria trinervia (Spreng.) C. Mohr. Planta 186, 304–312 (1992). https://doi.org/10.1007/BF00196260

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