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Transcriptional control of plastid gene expression during development of primary foliage leaves of barley grown under a daily light-dark regime

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Abstract

Plastid DNA transcription was studied during biogenesis and maturation of chloroplasts in primary foliage leaves of barley (Hordeum vulgare L.) seedlings grown under a daily light-dark regime. Specific age-dependent changes in the transcript pattern of plastids have been shown by hybridization to barley plastid DNA fragments of run-on transcript probes derived from homogeneous populations of proplastids and chloroplasts of different age. In proplastids, transcription of the rrn operon is predominant and the increasing transcriptional activity during biogenesis of chloroplasts is mainly the consequence of additional transcription of mRNA and tRNA genes. During maturation of chloroplasts, rrn transcription preferentially decreases and, conversely, transcription of the psbD gene increases. The importance of these changes in plastid DNA transcription is discussed in relation to chloroplast ageing.

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Abbreviations

bp:

base pairs

IR:

inverted repeat

kbp:

kilo base pairs

SSC:

small single-copy region

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  1. Karin Krupinska

    Present address: Institut für Allgemeine Botanik der Universität Hamburg, Ohnhorststrasse 18, W-2000, Hamburg 52, Federal Republic of Germany

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  1. Botanisches Institut der Universität Kiel, Olshausenstrasse 40, W-2300, Kiel 1, Federal Republic of Germany

    Karin Krupinska

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For his support of the project I would like to thank Professor K. Apel (ETH Zürich, Switzerland). I am grateful to A. Schmidt for excellent technical assistance. A. Bräutigam and C. Adami are thanked for drawing the figures and doing the photographic work. Dr. K. Humbeck and Dr. P. Lazzeri (Institut für Allgemeine Botanik, Universität Hamburg, Germany) are thanked for critical reading of the manuscript. This work was supported in part by the Bundesminister für Forschung und Technologie, Bonn.

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Krupinska, K. Transcriptional control of plastid gene expression during development of primary foliage leaves of barley grown under a daily light-dark regime. Planta 186, 294–303 (1992). https://doi.org/10.1007/BF00196259

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