Abstract
Steady-state 86Rb+ fluxes and contents were measured in stomatal guard cells of Vicia faba L., using “isolated” epidermal strips in which all cells other than the guard cells had been killed by a brief ultrasound treatment. Flux experiments were carried out for a range of stomatal apertures, achieved by incubation in light or dark, or on solutions containing different concentrations of RbCl (1–30 mM). At pH 5.5 the efflux curve of 86Rb+ could be fitted to the sum of two exponential terms and it was possible to calculate compartmental contents and fluxes. The data indicate that steady-state stomatal opening is achieved by regulation of both influx and efflux. Salt accumulation was not always adequate to contribute the observed osmotic requirement for opening, and this may indicate that some other solute accounts for the discrepancy.
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Abbreviations
- A:
-
amplitude
- k:
-
rate constant
- Mes:
-
2-(N-morpholino)-ethanesulphonic acid
- QT, QC, QV :
-
total, cytoplasmic, vacuolar chemical content
- Q* :
-
tracer content
- ØP, Øl :
-
plasmalemma, tonoplast fluxes
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This work was supported by a Research Studentship from the Science and Engineering Research Council. I thank Professor E.A.C. MacRobbie for much helpful discussion and advice.
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Brindley, H.M. Fluxes of 86Rb+ in “isolated” guard cells of Vicia faba L.. Planta 181, 432–439 (1990). https://doi.org/10.1007/BF00195898
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DOI: https://doi.org/10.1007/BF00195898