Abstract
Synechococcus PCC 6301 synthesized sucrose as a compatible solute following hyperosmotic shock induced by NaCl. Initial rates of photosynthetic 14C incorporation were reduced following salt shock. Photosynthetic rates were comparable in cells enriched for glycogen (by growth in NO -3 -deficient medium) and cells grown in NO -3 -sufficient medium in the absence of osmotic shock. Incorporation of 14C was predominantly into the NaOH fraction and the residual acidic fraction in cells grown in NO -3 -sufficient medium, whereas incorporation was predominantly into the residual acidic fraction in cells grown in NO -3 -deficient medium. Following salt stress, 14C incorporation was initially into the ethanol-soluble fraction and the majority of tracer was recovered in sucrose. Carbon-14 was detected in sucrose in cells which had been enriched for [14C]glycogen prior to salt stress, inferring that glycogen can act as a carbon source for sucrose synthesis following salt stress. Changes in the specific activity of sucrose are consistent with an initial synthesis of sucrose from glycogen followed by synthesis of sucrose using newly fixed carbon, in response to salt stress.
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References
Allen, M.M., Smith, A.J. (1969) Nitrogen chlorosis in blue-green algae. Arch. Mikrobiol. 69, 114–120
Blumwald, E., Tel-Or, E. (1984) Salt adaptation of the cyanobacterium Synechococcus 6311 growing in a continuous culture (turbidostat). Plant Physiol. 74, 183–185
Borowitzka, L.J., Demmerle, S., Mackay, M.A., Norton, R.S. (1980) Carbon 13 nuclear magnetic resonance study of osmoregulation in a blue-green alga. Science 210, 650–651
Erdmann, N. (1983) Organic osmoregulatory solutes in blue-green algae. Z. Pflanzenphysiol. 110, 147–155
Ernst, A., Böger, P. (1985) Glycogen accumulation and the induction of nitrogenase activity in the heterocyst-forming cyanobacterium Anabaena variabilis. J. Gen. Microbiol. 131, 3147–3153
Fry, I.V., Huflejt, M., Erber, W.W.A., Peshek, G.A., Packer, L. (1986) The role of respiration during adaptation of the freshwater cyanobacterium Synechococcus 6311 to salinity. Arch. Biochem. Biophys. 244, 686–691
Hagemann, M., Erdmann, N., Wittenburg, E. (1987) Synthesis of glucosylglycerol in salt-stressed cells of the cyanobacterium Microcystis firma. Arch. Microbiol. 148, 275–279
Kerby, N.W., Reed, R.H., Rowell, P. (1989) Separation of algal organic osmolytes by high-performance liquid chromatography. J. Chromatogr. 479, 353–360
Mackay, M.A., Norton, R.S. (1987) 13C nuclear magnetic resonance study of biosynthesis of glucosylglycerol by a cyanobacterium under osmotic stress. J. Gen. Microbiol. 133, 1535–1542
Mackinney, G. (1941) Absorption of light by chlorophyll solutions. J. Biol. Chem. 140, 315–322
Reed, R.H., Stewart, W.D.P. (1988) The responses of cyanobacteria to salt stress. In: Biochemistry of the algae and cyanobacteria, pp. 217–231, Rogers, L.J., Gallon, J.R., eds. Oxford University Press, Oxford
Reed, R.H., Warr, S.R.C., Richardson, D.L., Moore, D.J., Stewart, W.D.P. (1985) Multiphasic osmotic adjustment in a euryhaline cyanobacterium. FEMS Microbiol. Letts. 28, 225–229
Reed, R.H., Borowitzka, L.J., Mackay, M.A., Chudek, J.A., Foster, R., Warr, S.R.C., Moore, D.J., Stewart, W.D.P. (1986) Organic solute accumulation in osmotically stressed cyanobacteria, FEMS Microbiol. Rev. 39, 51–56
Reed, R.H., Kerby, N.W., Stewart, W.D.P. (1987) Particle size analysis of unicells formed by mild sonication of filaments of the blue-green alga Anabaena variabilis. Phycologia 26, 391–393
Rippka, R.J., Deruelles, J., Herdman, M., Waterbury, J.B., Stanier, R.Y. (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111, 1–61
Smith, A.J. (1982) Modes of cyanobacterial carbon metabolism. In: Biology of the cyanobacteria, pp. 47–85, Carr, N.G., Whitton, B.A. eds. Blackwell, Oxford
Stal, L.J., Reed, R.H. (1987) Low-molecular mass carbohydrate accumulation in cyanobacteria from a marine microbial mat in response to salt. FEMS Microbiol. Ecol. 45, 305–312
Tel-Or, E., Spath, S., Packer, L., Mehlhorn, R.J. (1986) Carbon-13 NMR studies of salt shock-induced carbohydrate turnover in the marine cyanobacterium Agmenellum quadruplicatum. Plant Physiol. 82, 646–652
Vonshak, A., Guy, R., Guy, M. (1988) the response of the filamentous cyanobacterium Spirulina platensis to salt stress. Arch. Microbiol. 150, 417–420
Warr, S.R.C. (1985) Low molecular weight solutes: their role in the osmotic adjustment of cyanobacteria. Ph.D. thesis, University of Dundee, UK
Warr, S.R.C., Reed, R.H., Stewart, W.D.P. (1984) Physiological responses of Nodularia harveyana to osmotic stress. Mar. Biol. 79, 21–26
Warr, S.R.C., Reed, R.H., Stewart, W.D.P. (1985a) Carbohydrate accumulation in osmotically stressed cyanobacteria (blue-green algae): interactions of temperature and salinity. New Phytol. 100, 285–292
Warr, S.R.C., Reed, R.H., Chudek, J.A., Foster, R., Stewart, W.D.P. (1985b) Osmotic adjustment in Spirulina platensis. Planta 163, 424–429
Warr, S.R.C., Reed, R.H., Stewart, W.D.P. (1987) Low-molecularweight carbohydrate biosynthesis and the distribution of cyanobacteria (blue-green algae) in marine environments. Br. Phycol. J. 22, 175–180
Warr, S.R.C., Reed, R.H., Stewart, W.D.P. (1988) The compatibility of osmotica in cyanobacteria. Plant Cell Environ. 11, 137–142
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This work was supported by the Agricultural and Food Research Council.
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Kerby, N.W., Reed, R.H. & Rowell, P. Incorporation of 14C in the cyanobacterium Synechococcus PCC 6301 following salt stress. Planta 181, 393–398 (1990). https://doi.org/10.1007/BF00195893
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DOI: https://doi.org/10.1007/BF00195893