Abstract
The immunological relationships between chlorophyll-a/b proteins from higher-plant thylakoid membranes have been studied by assaying purified chlorophyll proteins (CPs) with polyclonal and monoclonal antibodies. Although low levels of cross-reactions were observed between all light-harvesting proteins, the peripheral antennae (LHCII) were largely distinct from the inner antennae (CP 26 and CP 29). Chlorophyll-protein 24 and LHCI-680 have been proposed to have a role in connecting the inner and outer antennae, respectively, in photosystems I and II, and were closely related. The immunological relationships closely corresponded to the spectral properties. Antibodies were also used for locating chlorophyll-a/b proteins in grana, stroma and bundle-sheath membranes showing a strong lateral heterogeneity, which was maintained following State I State II transition. The only exception to this pattern was a specific LHCII population enriched in State-II stroma membranes. Chlorophyll proteins from bundlesheath chloroplasts, that have only cyclic electron flow, had epitopes distinct from those of their mesophyll homologues.
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Abbreviations
- Chl:
-
chlorophyll
- CP:
-
chlorophyll protein
- kDa:
-
kilodalton
- LHC:
-
light harvesting complex
- MW:
-
molecular weight
- PAGE:
-
polyacrylamide gel electrophoresis
- PSI:
-
photosystem I
- PSII:
-
photosystem II
- RC:
-
reaction center
- SDS:
-
sodium dodecyl sulphate
- α-CP 29:
-
antibodies raised against CP 29
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A preliminary report was presented at the VIII Int. Congr. on Photosynthesis, Stockholm 1989
We thank Professor G. Hoyer-Hansen (Carlsberg Research Center, Copenhagen, Denmark) and S. Darr (Department of Biology, Indiana University, Bloomington, USA) for their gift of the monoclonal antibodies. Dr. Roberto Barbato (Dipartimento di Biologia, Padua, Italy) is thanked for his assistance in the preparation of PSI-LHCI. We are grateful to Dr. Paola Dainese (Dipartimento di Biologia, Padua, Italy) for help in the purification of Chl-a/b complexes and to Dr. Francis-André Wollman (Institut de Biologie Physico-Chimique, Paris, France) for critically reading the manuscript.
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Di Paolo, M.L., Dal Belin Peruffo, A. & Bassi, R. Immunological studies on chlorophyll-a/b proteins and their distribution in thylakoid membrane domains. Planta 181, 275–286 (1990). https://doi.org/10.1007/BF00195877
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DOI: https://doi.org/10.1007/BF00195877