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DNA-ploidy, G2M-fractions and prognosis of stages B and C prostate carcinoma

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Abstract

Paraffin embedded tissue of 49 stage C and 27 stage B prostate adenocarcinomas was investigated by flow cytometry. All patients were treated by radical prostatectomy with pelvic lymphadenectomy and followed up for 5–10 years. The tumour was separated from the benign tissue to increase the proportion of tumour cells. Ten stage C and seven stage B carcinomas had to be excluded because of poor fixation. Six of the 39 (15%) stage C and 1/20 (5%) stage B carcinomas were aneuploid. Cell cycle analysis was done with correction for sliced nuclei and background subtraction. The threshold between carcinomas with low and with increased (“tetraploid”) G2M-fraction was determined by comparing carcinomas with and without tumour progression. Sixty-seven percent of the patients with non-euploid stage C carcinomas and 11% of those with euploid carcinomas suffered from tumour progression (P<0.01). The respective values for the stage B carcinomas were 67% and 6% (P<0.01). These results demonstrate the strong prognostic impact of DNA-ploidy and G2M-fractions for each individual patient.

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Authors and Affiliations

  1. Institute of Pathology, University of Würzburg, Josef-Schneider-Strasse 2, D-97080, Würzburg, Germany

    J. G. Müller, S. Demel & H. A. Müller

  2. Department of Urology, University of Würzburg, Würzburg, Germany

    M. P. Wirth, A. Manseck & H. G. W. Frohmüller

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  1. J. G. Müller
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  2. S. Demel
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  3. H. A. Müller
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  4. M. P. Wirth
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  5. A. Manseck
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  6. H. G. W. Frohmüller
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Müller, J.G., Demel, S., Müller, H.A. et al. DNA-ploidy, G2M-fractions and prognosis of stages B and C prostate carcinoma. Vichows Archiv A Pathol Anat 424, 647–651 (1994). https://doi.org/10.1007/BF00195780

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  • DOI: https://doi.org/10.1007/BF00195780

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