Abstract
This study documents the reactions of the monoclonal antibody KP-1, which detects histiocytes in paraffin sections, with 137 sarcomas, 48 lymphomas, 28 carcinomas, 7 malignant melanomas and 8 cystosarcoma phyllodes. The soft tissue sarcomas had been previously immunophenotyped. Positive staining was obtained in all categories of sarcoma except clear-cell sarcomas. Most categories of sarcoma showed staining in less than 10% of tumour cells although a minority of leiomyosarcomas showed more extensive staining. Five of 7 malignant melanomas were also positive while all lymphomas and carcinomas were negative. We conclude that KP-1 positivity is not helpful in supporting the histiocytic origin of a tumour and is of limited value in the differential diagnosis of soft tissue sarcomas or their separation from other categories of malignancy.
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Aoyama C, Hachitanda Y, Sato JK, Said JW, Shimada H (1991) Undifferentitated (embryonal) sarcoma of the liver. Am J Surg Pathol 15:615–624
Brandtzaeg P, Dole I, Gabrielsen T (1992) The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function. Histopathology 21:191–196
Facchetti F, Bertalot G, Grigolato PG (1991) KP-1 (CD68) staining of malignant melanomas. Histopathology 19:141–145
Falk S, Stutte HJ, Frizzera G (1991) Littoral cell angioma: a novel splenic vascular lesion demonstrating histiocytic differentiation. Am J Surg Pathol 15:1023–1033
Fletcher CDM (1992) Soft tissue tumours: an update. In: Anthony PP, MacSween RNM (eds) Recent advances in histopathology. Churchill Livingstone, London, pp 113–139
Horny H-P, Schaumburgheuer G, Bolz S, Geerts ML, Kaiserling E (1990) Use of monoclonal antibody KP1 for identifying normal and neoplastic human mast cells. J Clin Pathol 43:719–722
Iwasaki H, Yoshitake K, Ohijimi Y et al. (1992) Malignant fibrous histiocytoma: proliferative compartment and heterogeneity of “histiocytic” cells. Am J Surg Pathol 16:735–745
Leader M, Collins M, Patel J, Henry K (1986a) Staining for factor VIII related antigen and Ulex europeus agglutin I (UEA-I) in 230 tumours. An assessment of their specificity for angiosarcoma and Kaposi's sarcoma. Histopathology 10:1153–1162
Leader M, Collins M, Patel J, Henry K (1986b) Anti-neuron specific enolase staining reactions in sarcomas and carcinomas: its lack of neuroendocrine specificity. J Clin Pathol 39:1186–1192
Leader M, Patel J, Makin CAM, Henry K (1986c) An analysis of the sensitivity and specificity of the cytokeratin marker CAM 5.2 for epithelial tumours. Histopathology 10:1315–1324
Leader M, Patel J, Collins M, Henry K (1987a) Anti-alpha-1-antichymotrypsin staining of 194 sarcomas, 38 carcinomas and 17 malignant melanomas. Its lack of specificity as a tumour marker. Am J Surg Pathol 4:133–139
Leader M, Collins M, Patel J, Henry K (1987b) Desmin: its value as a marker of muscle derived tumours using a commercial antibody. Virchows Arch [A] 411:345–349
Leader M, Collins M, Patel J, Henry K (1987c) Vimentin: an evaluation of its role as a tumour marker. Histopathology 11:63–72
Leader M, Patel J, Henry K (1989) Myoglobin: an evaluation of its role as a marker of rhabdomyosarcomas. Br J Cancer 59:106–109
Loftus B, Loh LC, Curran B, Henry K, Leader M (1991) Mac 387: its non-specificity as a tumour marker or marker of histiocytes. Histopathology 19:251–255
Mackenzie DH (1970) The differential diagnosis of fibroblastic tumours. Blackwell Scientific, Oxford
Micklem K, Rigney E, Cordell J, et al. (1989) A human macrophage associated antigen (CD68) detected by 6 different monoclonal antibodies. Br J Haematol 73:6–11
Montgomery EA, Meis JM (1991) Nodular fasciitis: its morphologic spectrum and immunohistochemical profile. Am J Surg Pathol 15:942–948
Pulford KAF, Rigney EM, Micklem KJ, Jones M, Stross WP, Gatter KC, Mason DY (1989) KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol 42:414–421
Pulford KAF, Sipos A, Condell JL, Stross WP, Mason DY (1990) Distribution of the CD68 macrophage/myeloid associated antigen. Int Immunol 2:973–980
Rossi ML, Butler J, Heath SA (1991) Monocyte/macrophage infiltration in 41 oligoendrogliomas: paraffin was study. Path Res Pract 187:166–169
Smith MEF, Costa MJ, Weiss SW (1991) Evaluation of CD68 and other histiocytic antigens in angiomatoid malignant fibrous histiocytoma. Am J Surg Pathol 15:757–763
Soini Y, Miettinen M (1990) Immunohistochemistry of markers of histiomonocytic cells in malignant fibrous histiocytomas: a monoclonal antibody study. Path Res Pract 186:759–767
Swanson PE, Wick MR (1989) Clear cell sarcoma. An immunohistochemical analysis of six cases and comparison with other epithelioid neoplasms of soft tissue. Arch Pathol Lab Med 113:55–60
Tsang WYW, Chan JKC (1992) KP-1 (CD68) staining of granular cell neoplasms: is KP-1 a marker for lysosomes rather than the histiocytic lineage? Histopathology 21:84–86
Warnke RA, Pulford KAF, Pallesen G, Ralfkiaer E, Brown DC, Gatter KC, Mason DY (1989) Diagnosis of myelomonocytic and macrophage neoplasms in routinely processed tissue biopsies with monoclonal antibody KP1. Am J Pathol 135:1089–1095
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Cassidy, M., Loftus, B., Whelan, A. et al. KP-1: not a specific marker. Staining of 137 sarcomas, 48 lymphomas, 28 carcinomas, 7 malignant melanomas and 8 cystosarcoma phyllodes. Vichows Archiv A Pathol Anat 424, 635–640 (1994). https://doi.org/10.1007/BF00195778
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DOI: https://doi.org/10.1007/BF00195778