Abstract
The activity of α-amylase (EC 3.2.1.1) in mung bean (Vigna radiata (L.) Wilczek) cotyledons increased markedly in response to wounding. The changes in enzyme activity were in parallel with those in enzyme content. The level of α-amylase mRNA also notably increased in wounded cotyledons and attained its maximum level during the period between 1 and 2 d after wounding. The level of mRNA for phenylalanine ammonia-lyase, which is one of the well-characterized stress-inducible proteins, also increased after wounding, but the increase in mRNA level was faster than that of α-amylase mRNA. On the other hand, the content of mRNA for actin, a ‘housekeeping’ protein, was almost the same in wounded and unwounded cotyledons. The increase in α-amylase mRNA level in wounded cotyledons was severely inhibited by α-amanitin and cordycepin. α-Amylase expression in the first leaves of mung-bean seedlings was also induced by wounding.
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Abbreviations
- PAL:
-
phenylalanine ammonia-lyase
- SSC:
-
standard saline citrate
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We greatly acknowledge Prof. Richard Meagher, Department of Genetics, University of Georgia, Athens, USA for the gift of soybean actin gene clone. We also thank Mr. Kaoru Ishiwata for technical assistance.
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Koizuka, N., Tanaka, Y. & Morohashi, Y. Expression of α-amylase in response to wounding in mung bean. Planta 195, 530–534 (1995). https://doi.org/10.1007/BF00195711
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DOI: https://doi.org/10.1007/BF00195711