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Starch degradation in intact amyloplasts isolated from cauliflower floral buds (Brassica oleracea L.)

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Abstract

Isolated amyloplasts from cauliflower buds are capable of mobilizing starch. This mobilization is strongly dependent upon the intactness of the plastids and is linear with time for up to 30 min. The degradation of starch occurs via a hydrolytic breakdown and is stimulated by ATP-dependent phosphorylation of products of this hydrolysis. The rate of phosphorolytic stimulation of starch degradation is negligible. Carbohydrates derived from starch degradation do not appear to enter the oxidative pentose-phosphate pathway. Phosphorylation of hydrolytically solubilized intermediates leads to the synthesis of dihydroxyacetone phosphate and hexose phosphates. 3-Phosphoglyceric acid acts as an inhibitor of starch mobilization. The export of labelled phosphorylated intermediates from amyloplasts containing 14C-labelled starch implies the presence of an amyloplastic phosphate translocator in these plastids. The physiological role of varying concentrations of 3-phosphoglyceric acid is discussed with respect to the regulation of a metabolic cycle of simultaneous starch synthesis and degradation.

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Abbreviations

ADPGlcPPase:

ADPglucose pyrophosphorlyase

DHAP:

dihydroxyacetone phosphate

DIDS:

4,4′-diisothiocyano-2,2′-stilbene disulphonic acid

PGA:

3-phosphoglyceric acid

Glc6P:

glucose-6-phosphate

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Authors and Affiliations

  1. Pflanzenphysiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, D-49069, Osnabrück, Germany

    H.-Ekkehard Neuhaus, Gudrun Henrichs & Renate Scheibe

Authors
  1. H.-Ekkehard Neuhaus
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  2. Gudrun Henrichs
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  3. Renate Scheibe
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Correspondence to H.-Ekkehard Neuhaus.

Additional information

We thank Dr. Antje von Schaewen and Olaf Batz (Pflanzenphysiologie, Universität Osnabrück) for reading the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft, SFB 171-C16.

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Neuhaus, HE., Henrichs, G. & Scheibe, R. Starch degradation in intact amyloplasts isolated from cauliflower floral buds (Brassica oleracea L.). Planta 195, 496–504 (1995). https://doi.org/10.1007/BF00195706

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  • DOI: https://doi.org/10.1007/BF00195706

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