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Elevated temperature reduces starch deposition in wheat endosperm by reducing the activity of soluble starch synthase

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Abstract

Temperatures of more than 25° C adversely affect the activity of soluble starch synthase (SSS), an amyloplastic enzyme, in endosperm of wheat (Triticum aestivum L. cv. Mardler). Enzyme rate was found to have a temperature optimum between 20 and 25°C. This effect was apparently reversible after a short period of exposure to elevated temperature. We also found that with a prolonged period of exposure to elevated temperature there was another temperature-related phenomenon which caused a loss of enzyme activity that appeared to be much slower to reverse. We have termed this effect of temperature on SSS activity “knockdown”. The knockdown in SSS activity also occurred in-vivo. However, elevated temperature did not affect the activities of several other enzymes in the pathway of starch synthesis (ADP-glucose pyrophosphorylase, UDP-glucose pyrophosphorylase, sucrose synthase, phosphoglucomutase, phosphoglucose isomerase, bound starch synthase or hexokinase). Because the knockdown effect appeared to be specific to the enzyme SSS, we quantified the effect of knockdown on flux of carbon into starch and used these data to calculate the flux-control coefficient for SSS. Using data at 10–20°C the flux-control coefficient was CStarch10–20C = 0.50, whereas at 20–30° C the flux-control coefficient was CStarch20–30C = 1.38, and between 30–40°C the flux-control coefficient was CStarch30–40C = 0.69. Using data at 10–30°C the flux-control coefficient was CStarch10–30C = 1.15, and at 10–40°C the flux-control coefficient was CStarch10–40C = 0.82. In conclusion, we suggest that SSS is a major site of regulation of starch synthesis in developing wheat grain. During periods of high temperature the control point in the pathway of starch synthesis is apparently not associated exclusively with ADP-glucose pyrophosphorylase. In field conditions, in which temperatures are fluctuating, there will likely be periods of control of starch synthesis being exerted predominantly by SSS. During periods at lower temperature, control of flux may be exerted by SSS, perhaps in combination with other flux-controlling enzymes in the pathway. Our data point-out a crucial new aspect of quantifying control strengths of enzymes in plants: the determination of enzyme control strengths should be done in carefully regulated temperature conditions. Thus, since temperature is a major determinant of real flux through a pathway and the individual enzymes can respond differently to changing temperature conditions, the control strengths of individual steps in a pathway may vary with changing environmental conditions. This is particularly pronounced in starch deposition, because of the temperature instability of SSS.

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Abbreviations

ADP-Glc:

adenosine diphosphoglucose

SSS:

soluble starch synthase

UDP-Glc:

uridine diphosphoglucose

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Author notes

  1. P. L. Keeling

    Present address: Research Department, ICI Seeds, Applied Biology Project, PO Box 500, 50244, Slater, IA, USA

  2. P. J. Bacon

    Present address: ICI Chemical and Polymers Division, Runcorn, Cheshire, UK

  3. D. C. Holt

    Present address: Zeneca Seeds, Plant Biotechnology Section, Jealotts Hill Research Station, nr. Bracknell, Berkshire, UK

Authors and Affiliations

  1. ICI Seeds, Corporate Bioscience Laboratory, Runcorn, Cheshire, UK

    P. L. Keeling, P. J. Bacon & D. C. Holt

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  1. P. L. Keeling
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Additional information

We wish to thank Dr. Ian Bridges (Zeneca Seeds, Bracknell, UK) and Professor Tom ap Rees (Botany School, Cambridge University, UK) for their constructive criticism during the course of this work. We also wish to thank Dr. Kay Denyer (John Innes Centre, Norich, UK) for her helpful comments on this manuscript.

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Keeling, P.L., Bacon, P.J. & Holt, D.C. Elevated temperature reduces starch deposition in wheat endosperm by reducing the activity of soluble starch synthase. Planta 191, 342–348 (1993). https://doi.org/10.1007/BF00195691

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