Abstract
Salt-detergent extraction of purified plant nuclei yields a fraction enriched in putative structural proteins known as the “nuclear matrix”. Compared with mammalian nuclear matrices, which contain three major proteins, plant nuclear matrices are complex, containing at least 100 polypeptides. In order to characterise more fully the plant nuclear matrix we have used antibodies raised against both yeast (Saccharomyces cerevisiae) and mammalian (rat) nuclear pore proteins. We have shown that the nuclear matrix of carrot (Daucus carota L.) contains at least one nucleoporin-like protein of about 100 kDa which is immunologically related to both the yeast nuclear pore protein NSP1 and mammalian nucleoporins (p62). Antibody labelling of a variety of plant cells at the light-microscope and electron-microscope levels confirms that this antigen is located at the nuclear pores. This, to our knowledge, is the first identification of a nuclear pore protein in plants.
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Abbreviations
- IgG:
-
immunoglobulin G
- kDa:
-
kilodaltons
- DAPI:
-
4,6-diamidino-2-phenylindole
- FITC:
-
fluorescein isothioganate
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The authors would like to thank Dr. E. Hurt (European Molecular Biology Laboratory, Heidelberg, FRG) for antibodies against yeast nucleoporins, and Dr. L. Davis (Whitehead Institute for Biomedical Research, Cambridge, Mass., USA) for the monoclonal antibodies MAb 414 & 350. We thank Brian Wells for useful advice on electron microscopy. We also thank Peter Scott, Andrew Davis, and Nigel Hannant for photography, and Sue Bunnewell for development and printing of electronmicrographs.
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Scofield, G.N., Beven, A.F., Shaw, P.J. et al. Identification and localisation of a nucleoporin-like protein component of the plant nuclear matrix. Planta 187, 414–420 (1992). https://doi.org/10.1007/BF00195666
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DOI: https://doi.org/10.1007/BF00195666