Abstract
Differential screening of cDNA libraries from Ricinus communis L. cotyledons at the day 4 of germination against those at day 2 led to the isolation of two cDNA sequences which code for type C of the “nonspecific lipid-transfer protein”. The two clones are nearly identical in the coding region, which in each case starts with a sequence coding for a signal peptide. In the non-coding regions there is only 40% identity. Both clones are expressed only in the cotyledons and are strongly developmentally regulated, being exclusively expressed after the second day of germination with a maximum at days 4 and 5. No transcripts were found in the hypocotyls, roots or endosperm of seedlings and none in sink and source leaves of mature Ricinus plants.
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Abbreviations
- cDNA:
-
copy DNA tens
- LTP:
-
non-specific lipidtransfer protein
- nsLTPC:
-
non-specific lipid-transfer protein, type C
References
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The nucleotide-sequence data reported in this paper will appear in the EMBL, GenBank and DDBJ Nucleotide Sequence Databases under the accession numbers M86353 (nsLTPCl) and M86354 (nsLTPC2)
The discussion and experimental help by Dr. N. Sauer and Dr. W. Tanner (University of Regensburg, FRG) and Dr. C. Schobert (University of Bayreuth) are very gratefully acknowledged. The work was funded by Deutsche Forschungsgemeinschaft and Fonds der Chemischen Industrie.
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Weig, A., Komor, E. The lipid-transfer protein C of Ricinus communis L.: isolation of two cDNA sequences which are strongly and exclusively expressed in cotyledons after germination. Planta 187, 367–371 (1992). https://doi.org/10.1007/BF00195660
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DOI: https://doi.org/10.1007/BF00195660