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Characterization of a pollen-specific genomic clone from maize

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Summary

We have isolated a pollen-specific genomic clone of maize. This clone, designated Zmg13, was recovered by screening a maize genomic library with a pollen-specific maize cDNA clone. The coding region, and substantial portions of the 5′ and 3′ flanking regions, have been sequenced. The gene contains no introns. The sequence of the upstream region of the clone was used to search for homology with sequences from three other genes known to be expressed in pollen, although not pollen-specific. Several common sequence elements were identified, but whether these sequence elements are involved in pollen transcription remains to be determined. Restriction fragment length polymorphism mapping has shown the gene locus, designated mgs1, to be near the centromere on the short arm of chromosome 10.

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Hamilton, D.A., Bashe, D.M., Stinson, J.R. et al. Characterization of a pollen-specific genomic clone from maize. Sexual Plant Reprod 2, 208–212 (1989). https://doi.org/10.1007/BF00195580

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