Summary
We have isolated a pollen-specific genomic clone of maize. This clone, designated Zmg13, was recovered by screening a maize genomic library with a pollen-specific maize cDNA clone. The coding region, and substantial portions of the 5′ and 3′ flanking regions, have been sequenced. The gene contains no introns. The sequence of the upstream region of the clone was used to search for homology with sequences from three other genes known to be expressed in pollen, although not pollen-specific. Several common sequence elements were identified, but whether these sequence elements are involved in pollen transcription remains to be determined. Restriction fragment length polymorphism mapping has shown the gene locus, designated mgs1, to be near the centromere on the short arm of chromosome 10.
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References
Atchison ML (1988) Enhancers: mechanisms of action and cell specificity. Annu Rev Cell Biol 4:127–153
Burr B, Burr FA, Thompson KH, Albertson MC, Stuber CW (1988) Gene mapping with recombinant inbreds in maize. Genetics 118:519–526
Echt CS, Schwartz D (1981) Evidence for the inclusion of controlling elements within the structural gene at the waxy locus in maize. Genetics 99:275–284
Evola SV, Burr FA, Burr B (1986) The suitability of restriction fragment length polymorphisms as genetic markers in maize. Theor Appl Genet 71:765–771
Felder MR, Scandalios JR, Liu EH (1973) Purification and partial characterization of two genetically defined alcohol dehydrogenase isozymes in maize. Biochim Biophys Acta 317:149–159
Freeling M, Schwartz D (1973) Genetic relationships between the multiple alcohol dehydrogenases of maize. Biochem Genet 8:27–36
Frischauf AM, Lehrach H, Poustka A, Murray N (1983) Lambda replacement vectors carrying polylinker sequences. J Mol Biol 170:827–842
Hanson DD, Hamilton DA, Travis JL, Bashe DM, Mascarenhas JP (1989) Characterization of a pollen-specific cDNA clone from Zea mays and its expression. Plant Cell 1:173–179
Joshi CP (1987) An inspection of the domain between putative TATA box and translation start site in 79 plant genes. Nucleic Acid Res 15:6643–6653
Klosgen RB, Gierl A, Schwarz-Sommer Z, Saedler H (1986) Molecular analysis of the waxy locus of Zea mays. Mol Gen Genet 203:237–244
Lis JT (1980) Fractionation of DNA fragments by polyethylene glycol induced precipitation. Methods Enzymol 65:347–353
Maniatis T, Fritsch EF, Sambrook J (eds) (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Riven CJ, Zimmer EA, Walbot V (1982) Isolation of DNA and DNA recombinants from maize. In: Sheridan WF (ed) Maize for biological research. University Press, Grand Forks, ND, pp 161–164
Sachs MM, Dennis ES, Gerlach WL, Peacock WJ (1986) Two alleles of maize alcohol dehydrogenase 1 have 3′ structural and poly(A) addition polymorphisms. Genetics 113:449–467
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Stinson JR, Eisenberg AJ, Willing RP, Pe ME, Hanson DD, Mascarenhas JP (1987) Genes expressed in the male gametophyte of flowering plants and their isolation. Plant Physiol 83:442–447
Twell D, Wing R, Yamaguchi J, McCormick S (1989) Isolation and expression of an anther specific gene from tomato. Mol Gen Genet 217:240–245
Werr W, Frommer WB, Maas C, Starlinger P (1985) Structure of the sucrose synthase gene on chromosome 9 of Zea mays L. EMBO J 4:1373–1380
Willing RP, Bashe D, Mascarenhas JP (1988) An analysis of the quantity and diversity of messenger RNAs from pollen and shoots of Zea mays. Theor Appl Genet 75:751–753
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Hamilton, D.A., Bashe, D.M., Stinson, J.R. et al. Characterization of a pollen-specific genomic clone from maize. Sexual Plant Reprod 2, 208–212 (1989). https://doi.org/10.1007/BF00195580
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DOI: https://doi.org/10.1007/BF00195580