Abstract
⊎ Background: The spreading of epithelium is critical in the healing of corneal wounds. Such epithelial spreading requires the continuous production of protein and glycoprotein. To determine whether collagen production is required for the spreading of corneal epithelium, we studied the effects of inhibitors for collagen production on spreading of corneal epithelium in vitro. ⊎ Methods: We examined the effect of two proline analogs, l-azetidine 2-carboxylic acid and cis-hydroxyproline, a prolyl hydroxylase inhibitor, ethyl-3,4-dihydroxybenzoate, and a lysyl hydroxylase inhibitor, minoxidil, on the spreading of epithelium of organ-cultured rabbit cornea. ⊎ Results: Both analogs and inhibitors inhibited epithelial spreading in a dose-dependent manner. ⊎ Conclusion: These observations indicate that collagen production may be involved in the spreading of corneal epithelium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Carrington MJ, Fornieri C, Bartlet CP, Heale G, Levene CI (1985) Biochemical and ultrastructural study of the effects of proline analogues on collagen synthesis in 3T3 fibroblasts. J Pharm Pharmacol 37:794–801
Chung E-H, Wasson M, Zieske JD (1992) Expression of alpha enolase during corneal epithelial wound healing. Invest Ophthalmol Vis Sci [Suppl] 33:892
Gipson IK, Kiorpes TC (1982) Epithelial sheet movement: protein and glycoprotein synthesis. Dev Biol 92:259–262
Gipson IK, Riddle CV, Kiorpes TC, Spurr SJ (1983) Lectin binding to cell surfaces: comparison between normal and migrating corneal epithelium. Dev Biol 96:337–345
Gipson IK, Kiorpes TC, Brennan SJ (1984) Epithelial sheet movement: effect of tunicamycin on migration and glycoprotein synthesis. Dev Biol 101:212–220
Grotendorst GR, Seppä HE, Kleinman HK, Martin GR (1981) Attachment of smooth muscle cells to collagen and their migration toward platelet-derived growth factor. Proc Natl Acad Sci USA 78:3669–3672
Hashimoto K, Hatai M, Yaoi Y (1991) Inhibition of cell adhesion by type V collagen. Cell Struct Funct 16:391–397
Hashizume N, Saika S, Ooshima A (1993) Effects of collagens on corneal epithelial migration. (in Japanese) Atarashii Ganka 10:790–792
Ishizaki M, Westerhausen-Larson A, Kino J, Hayashi T, Kao WW-Y (1993) Distribution of collagen IV in human ocular tissues. Invest Ophthalmol Vis Sci 34:2680–2689
Konomi H, Hayashi T, Nakayasu K, Arima M (1984) Localization of type V collagen and type IV collagen in human cornea, lung, and skin. Am J Pathol 116:417–426
LeBaron RG, Höök A, Esko JD, Gay S, Höök M (1989) Binding of heparan sulfate to type V collagen. A mechanism of cell-substrate adhesion. J Biol Chem 264:7950–7956
Leushner JRA, Haust MD (1985) Glycoproteins on the surface of smooth muscle cells involved in their interaction with type V collagen. Can J Biochem Cell Biol 85:597–616
Linsenmayer TF, Fitch JM, Schmid TM, Zak NB, Gibney E, Sanderson RD, Mayne R (1983) Monoclonal antibodies against chicken type V collagen: production, specificity, and use for immunohistochemical localization in embryonic cornea and other organs. J Cell Biol 96:124–132
Linsenmayer TF, Fitch JM, Mayne R (1984) Extracellular matrices in the developing avian eye: type V collagen in corneal and noncorneal tissues. Invest Ophthalmol Vis Sci 25:41–47
Madri JA, Stern KS (1982) Aortic endothelial cell migration. I. Matrix requirements and composition. Am J athol 106:180–186
McAuslan BR, Reilly W, Hannan GN, Schindhelm K, Milthorpe B, Saur A (1988) Induction of endothelial cell migration by proline analogs and its relevance to angiogenesis. Exp Cell Res 176:248–257
Murad S, Pinnell SR (1987) Suppression of fibroblast proliferation and lysyl hydroxylase activity by minoxidil. J Biol Chem 262:11973–11978
Nishida T, Nakagawa S, Awata T, Ohashi Y, Watanabe K, Manabe R (1983) Fibronectin promotes epithelial migration of cultured rabbit cornea in situ. J Cell Biol 97:1653–1657
Ooshima A (1981) Collagen α B chain: increased proportion in human atherosclerosis. Science 213:666–668
Pedroza-Seres M, Vitale AT, Espaillat A, Lee SJ, Foster CS (1993) Distribution and expression of collagen types I and III in normal rat cornea and in wounded rat corneal epithelium: analysis by polymerase chain reaction. Invest Ophtbalmol Vis Sci 34:1318
Roll FJ, Madri JA, Albert J, Furthmayr H (1980) Codistribution of collagen types IV and AB2 in basement membranes and mesangium of the kidney. J Cell Biol 85:597–616
Sasaki T, Majamaa K, Uitto J (1987) Reduction of collagen production in keloid fibroblast cultures by ethyl-3,4-dihydroxybenzoate. Inhibition of prolyl hydroxylase activity as a mechanism of action. J Biol Chem 262:9397–9403
Stern KS, Madri JA, Roll FJ (1979) Migrating epidermis produces AB2 collagen and requires continual collagen synthesis for movement. Nature 277:229–232
Tang S-S, Trackman PC, Kagan HM (1982) Reaction of aortic lysyl oxidase with b-aminopropionitrile. J Biol Chem 258:4331–4338
Uitto J, Prockop DJ (1974) Incorporation of proline analogues into collagen polypeptides. Effects on the production of extracelluar procollagen and on the stability of the triple-helical structure of the molecule. Biochem Biophys Acta 336:234–251
Yeowell HN, Ha V, Walker LC, Murad S, Pinnell SR (1992) Characterization of a partial cDNA for lysyl hydroxylase from human skin fibroblasts: lysyl hydroxylase mRNAs are regulated differently by minoxidil derivatives and hydralazine. J Invest Dermatol 99:864–869
Zieske JD, Gipson IK (1986) Protein synthesis during corneal epithelial wound healing. Invest Ophthalmol Vis Sci 27:1–7
Zieske JD, Bukusogle G, Gipson IK (1989) Enhancement of vinculin synthesis by migrating stratified squamous epithelium. J Cell Biol 109:571–575
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Saika, S., Hashizume, N., Okada, Y. et al. Prolyl hydroxylase inhibitor and lysyl hydroxylase inhibitor inhibit spreading of corneal epithelium. Graefe's Arch Clin Exp Ophthalmol 232, 499–502 (1994). https://doi.org/10.1007/BF00195361
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00195361