Abstract
⊎ Background: Detection of different substances in aqueous humour is important for evaluation of the disorders affecting the eye. The purpose of the present study was to apply highresolution proton (1H) nuclear magnetic resonance (NMR) spectroscopy for extensive characterisation of the metabolites in the aqueous humour from rabbits. ⊎ Methods: High-resolution 1H NMR spectroscopy, including two-dimensional shift correlated (COSY) technique, was performed on aqueous humour from rabbits. ⊎ Results: More than 20 metabolites were simultaneously detected and identified in high-resolution 1H NMR spectra of aqueous humour from rabbits. Some of these were also quantified. ⊎ Conclusion: High-resolution 1H NMR spectroscopy is a valuable method for simultaneous detection of many different metabolites in aqueous humour.
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Gribbestad, I.S., Midelfart, A. High-resolution 1H NMR spectroscopy of aqueous humour from rabbits. Graefe's Arch Clin Exp Ophthalmol 232, 494–498 (1994). https://doi.org/10.1007/BF00195360
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DOI: https://doi.org/10.1007/BF00195360