, Volume 9, Issue 4, pp 190–194 | Cite as

Hydrogen fluoride effects on plasma membrane composition, ATPase activity and cell structure in needles of eastern white pine (Pinus strobus) seedlings

  • Krzysztof J. Rakowski
  • Janusz J. Zwiazek
  • Michael J. Sumner


Eastern white pine (Pinus strobus L.) seedlings were grown in controlled environment growth cabinets and fumigated with 0.4 and 1.6 μg m−3 hydrogen fluoride for 2–28 days. Plasma membranes were isolated from needles of treated and control seedlings and their chemical composition and ATPase activity examined to determine early effects of hydrogen fluoride action. In plants treated for 2 days with both fluoride levels, ratios of plasma membrane free sterols:phospholipids and sterols:proteins were drastically higher than ratios in control plants. Seedlings treated with hydrogen fluoride for 8 days contained plasma membranes with elevated phospholipid:protein and sterol:protein ratios and their plasma membrane ATPase activity was higher than that of control plants. Prolonged, 28-day hydrogen fluoride treatment with 1.6 μg m−3 level was the only treatment which produced a drastic inhibition of plasma membrane ATPase activity. During the initial stages of hydrogen fluoride treatment, treated cells did not show alterations of ultrastructure which were previously shown in cells of plants treated with soil applied sodium fluoride. The results of the present study indicate that the plasma membranes may be among the initial sites of hydrogen fluoride injury to plants as well as initial sites of defense reaction.

Key words

Plasma membrane Fluoride ATPase Sterols 


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  1. Gallagher SR, Leonard RT (1982) Effect of vanadate, molybdate, and azide on membrane-associated ATPase and soluble phosphatase activities of corn roots. Plant Physiol 70: 1335–1340Google Scholar
  2. Giannini JL, Pushnik JC, Briskin DP, Miller GW (1987) Fluoride effects on plasma membrane ATPase of sugarbeet. Plant Sci 53: 39–44Google Scholar
  3. Grandmougin A, Benveniste P, Hartman MA (1989) Effect of sterols on reconstituted plasma membrane H+-ATPase from maize roots. In: Dainty J, DeMichelis MI, Marre E, Rasi-Caldogno F (eds) Plant membrane transport: the current position, Elsevier, Amsterdam, pp 111–114Google Scholar
  4. Hellergren J, Widell S, Lundborg T, Kylin A (1983) Frosthardiness development in Pinus sylvestris: The involvement of a K+-stimulated Mg2+-dependent ATPase from purified plasma membranes of pine. Physiol Plant 58: 7–12Google Scholar
  5. McCune DC, Weinstein LH, Jacobson JS, Hitchock AE (1964) Some effects of atmospheric fluoride on plant (Milo maize, Phaseolus vulgaris) metabolism. J Air Pollut Control Assoc 14: 465–468Google Scholar
  6. McQuaker NR, Gurney M (1977) Determination of total fluoride in soil and vegetation using alkali fusion-selective ion electrode techniques. Analyt Chem 49: 53–56Google Scholar
  7. Palmgren MG (1991) Regulation of plant plasma membrane H+- ATPase activity. Physiol Plant 83: 314–323Google Scholar
  8. Palmgren MG, Sommarin M, Ulvskov P, Larsson C (1990) Effect of detergents on the H+-ATPase activity of inside-out and right-side-out plant plasma membrane vesicles. Biochim Biophys Acta 1021: 133–140Google Scholar
  9. Palta JP, Li PH (1980) Alteration in membrane transport properties by freezing injury in herbaceous plants: evidence against rupture theory. Physiol Plant 50: 169–175Google Scholar
  10. Peterson GL (1977) A simplification of the protein assay method of Lowry et al. which is generally more applicable. Anal Biochem 83: 346–351PubMedGoogle Scholar
  11. Rakowski KJ, Zwiazek JJ (1992) Early effects of hydrogen fluoride on water relations, photosynthesis and membrane integrity in eastern white pine (Pinus strobus) seedlings. Environ Exp Bot 32: 377–382Google Scholar
  12. Sailerova E, Zwiazek JJ (1993) Effects of triadimefon and osmotic stress on plasma membrane composition and ATPase activity in white spruce (Picea glauca) needles. Physiol Plant 87: 475–482Google Scholar
  13. Sandstrom RP, Cleland RE (1989) Comparison of the lipid composition of oat root and coleoptile plasma membranes. Lack of short-term change in response to auxin. Plant Physiol 90: 1207–1213Google Scholar
  14. Thorne JH (1985) Phloem unloading of C and N assimilates in developing seeds. Annu Rev Plant Physiol 36: 317–343Google Scholar
  15. Treshow M, Anderson FK (1989) Plant stress from air pollution. Wiley, TorontoGoogle Scholar
  16. Warburg O, Christian W (1942) Isolierung und Kristllisation des Garunsgsferment Enolase. Biochem Z 310: 385–421Google Scholar
  17. Weinstein LH (1977) Fluoride and plant life. J Occup Med 19: 49–78Google Scholar
  18. Widell S, Larsson C (1990) A critical evaluation of markers. In: Larsson C, Moller IM (eds) The plant plasma membrane, Springer, Berlin Heidelberg New York, pp 16–43Google Scholar
  19. Zwiazek JJ, Blake TJ (1990) Effects of preconditioning on electrolyte leakage and lipid composition in black spruce (Picea mariana) stressed with polyethylene glycol. Physiol Plant 79: 71–77Google Scholar
  20. Zwiazek JJ, Shay JM (1987) Fluoride- and drought-induced structural alterations of mesophyll and guard cells in cotyledons of jack pine (Pinus banksiana) Can J Bot 65: 2310–2317Google Scholar
  21. Zwiazek JJ, Shay JM (1988a) Sodium fluoride induced metabolic changes in jack pine seedlings. I. Effect on gas exchange, water content and carbohydrates. Can J For Res 18: 1305–1310Google Scholar
  22. Zwiazek JJ, Shay JM (1988b) Sodium fluoride induced metabolic changes in jack pine seedlings. II. Effect on growth, acid phosphatase, cytokinins, and pools of soluble proteins, amino acids, and organic acids. Can J For Res 18: 1311–1317Google Scholar
  23. Zwiazek JJ, Shay JM (1988c) The effects of sodium fluoride on cytoplasmic leakage and the lipid and fatty acid composition of jack pine (Pinus banksiana) seedlings. Can J Bot 66: 535–541Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Krzysztof J. Rakowski
    • 1
  • Janusz J. Zwiazek
    • 2
  • Michael J. Sumner
    • 3
  1. 1.Department of Genetics and Forest Trees PhysiologyForest Research InstituteWarsawPoland
  2. 2.Department of Forest ScienceUniversity of AlbertaEdmontonCanada
  3. 3.Department of BotanyUniversity of ManitobaWinnipegCanada

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