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Scale-associated glycoproteins of Scherffelia dubia (Chlorophyta) form high-molecular-weight complexes between the scale layers and the flagellar membrane

Planta volume 199, pages 503–510 (1996)Cite this article

Abstract

Flagellar scales were isolated from the flagellate green alga Scherffelia dubia. The flagellar scales consist mainly of acidic polysaccharides (70%) and glycoproteins (10%), and monosaccharide analyses show that the scales contain high amounts of unusual 2-keto-sugar acids. Approximately, 72 mol% of total carbohydrate is 3-deoxy-manno-2-octulosonic acid, 3-deoxy-5-O-methylmanno-2-octulosonic acid and 3-deoxy-lyxo-2-heptulosaric acid. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed the presence of at least 18 different scale-associated proteins (SAPs), ranging in apparent molecular mass from 77 kDa to over 300 kDa. Lectin blot analyses performed in combination with glycosidase treatment, showed that SAPs contained N-glycans of the highmannose type and the hybrid type, as well as a complex type that was not immunologically related to higher-plant complex glycans. Most of the SAPs were present in two or possibly three high-molecular-weight complexes. In these complexes, individual polypeptides are cross-linked by disulfide bridges. A polyclonal antibody was raised against a SAP of 126 kDa (SAP 126), a glycoprotein present in a high-molecular-weight complex. The SAP126 antibody was used to localize the protein between scale layer and flagellar membrane. We suggest that these high-molecular-weight complexes link scales to the flagellar membrane.

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Abbreviations

AAA:

Aleuria aurantia agglutinin

DSA:

Datura stramonium agglutinin

DTT:

dithiothreitol

GNA:

Galanthus nivalis agglutinin

RCA:

Ricinus communis agglutinin

SAP:

Scale-associated protein

TBS:

Tris-buffered saline

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Authors and Affiliations

  1. Botanisches Institut, Lehrstuhl I, Universität zu Köln, Gyrhofstrasse 15, D-50931, Köln, Germany

    Burkhard Becker, Lara Perasso, Andreas Kammann, Markus Salzburg & Michael Melkonian

Authors
  1. Burkhard Becker
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  2. Lara Perasso
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  3. Andreas Kammann
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  4. Markus Salzburg
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  5. Michael Melkonian
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Corresponding author

Correspondence to Burkhard Becker.

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Dedicated to Professor Eberhard Schnepf on the occasion of his 65th birthday.

This work was supported by the Deutsche Forschungsgemeinschaft and an Alexander von Humboldt Foundation research award to L. Perasso. We thank G. Noat for providing the anti-β-glucosidase and anti-pectin methyl esterase antibodies.

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Becker, B., Perasso, L., Kammann, A. et al. Scale-associated glycoproteins of Scherffelia dubia (Chlorophyta) form high-molecular-weight complexes between the scale layers and the flagellar membrane. Planta 199, 503–510 (1996). https://doi.org/10.1007/BF00195179

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  • DOI: https://doi.org/10.1007/BF00195179

Key words

  • Flagellar membrane
  • Glycoprotein immunolocalization
  • Lectin
  • Scale
  • Scherffelia