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Regulation of the low-CO2-inducible polypeptides in Chlamydomonas reinhardtii

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Abstract

Polypeptides of 21, 36 and 37 kDa are induced in the unicellular green alga Chlamydomonas reinhardtii Dang. when cells are transferred from high (2%) to low (0.03%) CO2 concentrations. The synthesis of these polypeptides is correlated with the induction of the CO2-concentrating mechanism. In this work we studied the effect of the growth conditions on the synthesis of these polypeptides with the aim of clarifying whether the induction of all three of these low-CO2-inducible polypeptides requires the same environmental factor. Our results showed that induction of the 21- and 36-kDa polypeptides under low-CO2 conditions occurred only in the light, while the 37-kDa periplasmic carbonic anhydrase (EC 4.2.1.1) was induced in light, darkness, and in both synchronous and asynchronous cultures. In addition, induction of these polypeptides appeared to be determined more by the O2/CO2 ratio than by the CO2 concentrations. None of these polypeptides could be induced in either of two different mutants of C. reinhardtii, one lacking ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) and the other with inactive enzyme. Our results indicate that the 21- and 36-kDa polypeptides are regulated by a mechanism different from that controlling the 37-kDa polypeptide.

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Abbreviations

pCA:

(periplasmic) carbonic anhydrase

Rubisco:

ribulose-1,5-bisphosphate carboxylase/oxygenase

TAP:

Trisacetate phosphate medium

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Authors and Affiliations

  1. Institute of Applied Algology, Instituto Tecnológico de Canarias, Muelle de Taliarte s/n, E-35214, Telde, Canary Islands, Spain

    Arsenio Villarejo, Guillermo García Reina & Ziyadin Ramazanov

  2. Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, E-28049, Madrid, Spain

    Arsenio Villarejo

Authors
  1. Arsenio Villarejo
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  2. Guillermo García Reina
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  3. Ziyadin Ramazanov
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Corresponding author

Correspondence to Ziyadin Ramazanov.

Additional information

The authors thank Prof. M. Spalding (Iowa State University, USA) for providing antisera to LIP-21 and LIP-36. We thank Prof. S. Bartlett and Dr. J. Moroney (Louisiana State University, USA) for providing antibodies to C. reinhardtii, Rubisco and 37-kDa pCA, respectively. This work was supported by the Instituto Tecnologico de Canarias.

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Villarejo, A., Reina, G.G. & Ramazanov, Z. Regulation of the low-CO2-inducible polypeptides in Chlamydomonas reinhardtii . Planta 199, 481–485 (1996). https://doi.org/10.1007/BF00195176

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  • DOI: https://doi.org/10.1007/BF00195176

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