Abstract
The distribution of keratins in N-methyl-N'-nitro-N-nitrosoguanidine-induced oesophageal carcinomas in shrews was tested immunohistochemically, using a panel of seven different monoclonal antibodies. The studies were done on methacarn-fixed paraffin-embedded tissue, using the labelled streptavidin biotin method, and the relationship between morphological characteristics and keratin reaction patterns in carcinomas was analysed and compared with that in adjacent “normal” oesophageal epithelium. In the normal oesophageal epithelia, KL1, AE1, AE3, CK8.12 and CK4.62 stained suprabasal cells, 312C8-1 reacted to basal cells, and KS-1A3 labelled all epithelial cells. In squamous cell carcinomas, almost all the cancer cells were labelled strongly by 312C8-1 and weakly by KS-1A3, while a few cells in the centres of the keratinized foci were stained by KL1, AE1, AE3, CK8.12, and CK4.62. Like human oesophageal carcinomas, shrew oesophageal carcinomas maintain expression of human keratin 14, as determined by 312C8-1. The expression of human keratin 13, as determined by KS-1A3, was down-regulated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adamson RH, Sieber SM (1983) Chemical carcinogenesis studies in nonhuman primates. In: Langenbach R, Nesnow S, Rice J (eds) Organ and species specificity in chemical carcinogenesis. Plenum, New York, pp 129–156
Banks-Schlegel SP, Harris CC (1983) Tissue specific expression of keratin proteins in human esophageal and epidermal epithelium and their cultured keratinocytes. Exp Cell Res 146:271–280
Cooper D, Schermer A, Sun T-T (1985) Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications, and limitations. Lab Invest 52:243–256
Grace MP, Kim KH, True LD, Fuchs E (1985) Keratin expression in normal esophageal epithelium and squamous cell carcinoma of the esophagus. Cancer Res 45:841–846
Herrold KM (1966) Epidermoid carcinomas of esophagus and forestomach induced in syrian hamsters by N-nitro-N-methyl-urethance. J Natl Cancer Inst 37:389–394
Huszar M, Gigi-Leitner O, Moll RW, Franke WW, Geiger B (1986) Monoclonal antibodies to various acidic (type I) cytokeratins of stratified epithelia. Differentiation 31:141–153
Iizuka T, Ichimura S, Kawachi T (1980) Esophageal carcinoma in rats induced by N-amyl-N-methylnitrosamine. Gann 71:94–99
Levy R, Czernobilsky B, Geiger B (1988) Subtyping of epithelial cells of normal and metaplastic human uterine cervix, using polypeptide-specific cytokeratin antibodies. Differentiation 39:185–196
Malecha MJ, Miettinen M (1991) Expression of keratin 13 in human epithelial neoplasms. Virchows Arch [A] 418:249–254
Matsuzaki T, Tanaka T, Saito R, Yamanaka M, Saito M, Nomura T (1992) Establishment of an outbred strain (Jic: SUN) in the house musk shrew, Suncus murinus. Exp Anim 41:167–172
Moll R, Franke WW, Schiller DL, Geiger B, Krepler R (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24
Moll R, Krepler R, Franke WW (1983) Complex cytokeratin polypeptide patterns observed in certain human carcinomas. Differentiation 23:256–269
Moses FM (1991) Squamous cell carcinoma of the esophagus. Natural history, incidence, etiology, and complications. Gastroenterol Clin North Am 20:703–716
Napalkov NP, Pozharisski KM (1969) Morphogenesis of experimental tumors of the esophagus. J Natl Cancer Inst 42:922–940
Romer AS, Parson TS (1978) The vertebrate body, 5th edn. Saunders, Philadelphia, pp 75–91
Sander J, Schweinsberg F (1973) Tumorinduktion bei Mäusen durch N-methylbenzyl-nitrosamin in niedriger Dosierung. Z Krebsforsch 79:157–161
Takubo K, Shisa H, Futatsuki K, Sasajima K (1981) Early changes of dog esophageal mucosa induced by N-ethyl-N'-nitro-N-nitrosoguanidine. Gann 72:880–885
Tsubura A, Hatano T, Hayama S, Morii S (1991) Immunophenotypic difference of keratin expression in normal mammary glandular cells from five different species. Acta Anat (Basel) 140:287–293
Tsubura A, Oyaizu T, Fujita Y, Asada M, Hilgers J, Morii S (1993a) Keratin phenotypes in basal cell carcinomas of the skin. Int J Oncol 2:431–435
Tsubura A, Senzaki H, Takahashi H, Oyaizu T, Morii S (1993b) Esophageal carcinoma in house musk shrews, Suncus murinus (insectivora), induced by N-methyl-N-nitro-N-nitrosoguanidine. J Cancer Res Clin Oncol 119:717–720
Viac J, Reano A, Brochier J, Staquet M-J, Thivolet J (1983) Reactivity pattern of a monoclonal antikeratin antibody (KL1). J Invest Dermatol 81:351–354
Wu Y, Chen J, Ohshima H, Pignatelli B, Boreham J, Li J, Campbell TC, Peto R, Bartsch H (1993) Geographic association between urinary excretion of N-nitroso compounds and oesophageal cancer mortality in China. Int J Cancer 54:713–719
Yang K, Lipkin M (1990) AE1 cytokeratin reaction patterns in different differentiation states of squamous cell carcinoma of the esophagus. Am J Clin Pathol 94:261–269
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Takahashi, H., Shikata, N. & Tsubura, A. Immunohistochemical reaction patterns of keratins in MNNG-induced shrew oesophageal carcinomas. Vichows Archiv A Pathol Anat 424, 267–271 (1994). https://doi.org/10.1007/BF00194610
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00194610