Abstract
The distribution of three cross-reactive materials (CRMs), a toxic protein analogous to canatoxin, CNTX-CRM, a lectin analogous to concanavalin A, Con A-CRM, and a major storage protein, canavalin-CRM, was investigated during successive stages of maturation of Canavalia brasiliensis Mart. seeds. The data obtained by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunological analyses indicated that these proteins share extensive homology with the analogous proteins found in Canavalia ensiformis seeds. The changes in CNTX-CRM and Con A-CRM levels throughout the maturation process were assayed by rocket immunoelectrophoresis. Synthesis of Con A-CRM was detectable at 30 days post-anthesis (DPA) while its hemagglutinating activity appeared only at 35 DPA. The CNTX-CRM was detected as a biologically active protein from 30 DPA onwards. The behavior of CNTX-CRM during maturation of C. brasiliensis seeds was quite distinct from that of Con A-CRM, pointing to different biological roles of these proteins in the seed.
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Abbreviations
- CNTX:
-
canatoxin
- Con A:
-
concanavalin A
- CRM:
-
cross-reactive material
- DPA:
-
days post-anthesis
- ev :
-
endovenous
- RIE:
-
rocket immunoelectrophoresis
- SDS-PAGE:
-
polyacrylamide gel electrophoresis in the presence of sodium docecyl sulphate
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This work was supported by Conselho Nacional de Desenvolvimento Cientifico e Tecnológico; Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior; Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro; Financiadora de Estudos e Projetos; Fundação Banco do Brasil, Brazil, and International Foundation for Science, Sweden. G.B. and C.R.C. are gratefull to Ms. Dione Maria da Silva and Francisco Gomes Neto for their technical assistance.
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Barcellos, G.B.S., Almeida, L.M., Moreira, R.A. et al. Canatoxin-, concanavalin A- and canavalin-cross-reactive materials during maturation of Canavalia brasiliensis (Mart.) seeds. Planta 189, 397–402 (1993). https://doi.org/10.1007/BF00194437
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DOI: https://doi.org/10.1007/BF00194437
Key words
- Canatoxin
- Canavalia
- Concanavalin A
- Canavalin
- Seed maturation