Abstract
The infection of cucumber leaves by Colletotrichum lagenarium was studied using cytological methods. Its progress in untreated plants was compared with that in plants in which systemic resistance had been induced by pre-infecting the first true leaf with the same fungus. In induced plants, a reduction of fungal development was observed at the leaf surface, in the epidermis, and in the mesophyll. On the leaf surface, formation of appressoria was slightly reduced. In the epidermis, enhanced formation of papillae beneath appressoria, and possibly increased lignification of entire cells, correlated with reduced development of infection hyphae. Papillae contained callose, identified by staining with aniline-blue fluorochrome and digestion with β-1,3-glucanase, as a main structural component. In the mesophyll, reduced fungal development provided evidence for the existence of an additional induced defence reaction. The results imply that preinfection elicited a systemic, multicomponent defence reaction of the host plant against the fungus.
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Abbreviations
- CI:
-
colour index
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Dedicated to the memory of Professor H. Grisebach
We thank Professor A. Slusarenko for critically reading the manuscript and P.D. R. Honegger for her help with the scanning electron microscope (both Institute of Plant Biology, University of Zürich). The work was supported by a grant from the Roche-Maag corporation (Dielsdorf, Switzerland).
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Kováts, K., Binder, A. & Hohl, H.R. Cytology of induced systemic resistance of cucumber to Colletotrichum lagenarium . Planta 183, 484–490 (1991). https://doi.org/10.1007/BF00194268
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DOI: https://doi.org/10.1007/BF00194268