Abstract
Sensitivity of herpes simplex virus (HSV) isolates, from patients with recurrent infections, to human fibroblast β interferon (IFN), was tested in vitro. Among 25 HSV strains, 12 were HSV-1, isolated from facial and labial lesions, and 13 were HSV-2 isolated from genital lesions.
Viral sensitivity to IFN was examined on epithelial MB cell line by yield reduction and plaque reduction and close correspondence between the two methods was observed. Most of HSV-1 isolates were in the same range of sensitivity to IFN, while HSV-2 isolates varied in sensitivity and differences approached statistical significance (P = 0.14). No correlation was found between various biological properties, such as plaque size, virulence in baby mice, growth at different temperatures, thymidine kinase activity in the presence or absence of IFN and sensitivity to IFN. Application of β IFN containing cream reduced clinical symptoms in most of the patients. However, larger numbers of patients should be evaluated in order to conclude whether in vitro sensitivity correlates to clinical improvement.
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References
Armstrong JA (1984) Clinical use of interferons. In: Came PE, Carter WA (eds) Interferons, Springer-Verlag, Berlin Heidelberg, pp 455–469
Armstrong JA, Skicki-Mullen MB, Breinig MK, Ho M (1983) Interferon susceptibility of herpes simplex virus strains isolated from patients enrolled in clinical trials. Antimicrob Agents Chemother 24:137–139
De Clercq E, Descamps J, Verhelst G, Walker RT, Jones AS, Torrence OP, Shugar D (1980) Comparative efficacy of anti herpes drugs against different strains of herpes simplex virus. J Inf Dis 141:563–574
Dix RD, McKendall RR, Baringer JR (1983) Comparative neurovirulence of Herpes Simplex Virus type 1 strains after peripheral or intracerebral inoculation of BALB/C mice. Infect Immun 40:103–112
Eisinger M, Kucarova O, Karkar NH, Good RA (1975) Propagation of human wart virus in tissue culture. Nature 256:432–434
Gentry GA, Allen GP, Holton R, Nevins RB, McGowan JJ, Veerisetty V (1983) Thymine salvage, mitochondria and the evolution of the herpes-viruses. Intervirology 19:76
Imanishi I, Matsubara M, Kishida T, Ozaki Y, Kurinura T (1980) Comparative studies on the inhibitory effects of interferon on various strains of herpes simplex viruses in vitro. Biken J 23:107–111
Katz E, Margalith E (1982) Characterization of an herpes simplex virus type 2 mutant which is resistant to acycloguanosine and causes fusion of BSc-1 cells. Arch Virol 74:111–123
Lazar R, Breinig MK, Armstrong JA, Ho M (1980) Response of cloned progeny of clinical isolates of herpes simplex virus to human leukocyte interferon. Infect Immun 28:708–712
Lerner AM, Bailey EJ (1976) Differential sensitivity of herpes simplex virus type 1 and 2 to human interferon: Antiviral effect of interferon plus 9-B-D. arabinofuranosyladenine. J Inf Dis 134:400–404
Leventon-Kriss S, Rannon L, Smetana Z, Yoffe R (1982) Genital herpes simplex infections in Israel; 1973 through 1980. Isr J Med Sci 18:942–946
Levin MK, Leary PL (1981) Inhibition of human herpes viruses by combination of acyclovir and human leukocyte interferon. Infect Immun 32:995–999
Mayo DR (1982) Differentiation of herpes simplex virus types 1 and 2 by sensitivity of (E)-5 (2-bromovinyl)-2′-deoxyuridine. J Clin Microbiol 15:733–736
Movshovitz M, Leventon-Kriss S, Shewach-Millet M, Doerner T (1984) Topical treatment with human fibroblast (beta) interferon cream in recurrent dermal and genital herpes simplex infection. Antiv Res 3:141
Overall J, Yeh TJ, Kern ER (1980) Sensitivity of herpes simplex virus types 1 and 2 to three preparations of human interferon. J Inf Dis 142:943
Otsuka H, Qari H, Kit S (1982) Inhibition by interferon of biochemical transformation induced by cloned herpes virus thymidine kinase genes. Antiv Res 2:301–311
Rapp F, Nashiyama Y (1980) Inhibition of herpes simplex virus type 2 induced biochemical transformation by interferon. J Virol 33:543–546
Rasmussen L, Farley LB (1975) Inhibition of herpes virus hominis replication by human interferon. Infect Immun 12:104–108
Stanwick TL, Shinazi RF, Campbell DE, Nahmias AJ (1981) Combined antiviral effect of interferon and acyclovir on herpes simplex virus types 1 and 2. Antimicr Agents Chemother 19:672–674
Weck PK, Came PE (1984) Comparative biologic acitivities of human interferons. In: Came PE, Carter WA (eds) Interferons. Springer, Berlin Heidelberg New York, pp 339–356
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Leventon-Kriss, S., Movshovitz, M., Smetana, Z. et al. Sensitivity in vitro of herpes simplex virus isolates to human fibroblast interferon. Med Microbiol Immunol 176, 151–159 (1987). https://doi.org/10.1007/BF00193896
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DOI: https://doi.org/10.1007/BF00193896