Skip to main content
SpringerLink
Log in

Isolation of two antigens from the culture filtrates of Mycobacterium tuberculosis and their applications in the laboratory diagnosis of the tuberculous meningitis

  • Original Investigations
  • Published:
Medical Microbiology and Immunology Aims and scope Submit manuscript

Abstract

Two antigens were isolated from the culture filtrates of H37Ra Mycobacterium tuberculosis by immunoabsorbent affinity chromatography, M. tuberculosis antigen 5 and immunoabsorbent affinity column-purified antigen (IAP). The potential application of these two mycobacterial antigens in the laboratory diagnosis of tuberculous meningitis was evaluated by indirect enzyme-linked immunoabsorbent assay in cerebrospinal fluid specimens. IAP antigen was more sensitive than antigen 5, although antigen 5 was more specific than IAP antigen in detecting tuberculous aetiology. Technical aspects of immunoabsorbent affinity chromatography have been highlighted in this study.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Coovadia YM, Dawood A, Ellis ME, Coovadia HM, Daniel TM (1986) Evaluation of adenosine deaminase activity and antibody to Mycobacterium tuberculosis antigen 5 in cerebrospinal fluid and the radioactive bromide partition test for the early diagnosis of tuberculosis meningitis. Arch Dis child 61:428–435

    Google Scholar 

  2. Daniel TM, Anderson PA (1977) The use of immunoabsorbents for the purification of mycobacterial antigens. J Lab Clin Med 90:354–360

    Google Scholar 

  3. Daniel TM, Anderson PA (1978) Isolation by immunoabsorbent affinity chromatography and physicochemical characterization of Mycobacterium tuberculosis antigen 5. Am Rev Respir Dis 117:533–539

    Google Scholar 

  4. Daniel TM, Janicki BW (1978) Mycobacterial antigens: a review of their isolations, chemistry and immunological properties. Microbiol Rev 42:84–113

    Google Scholar 

  5. Daniel TM, Ellner JJ, Todd LS, McCoy DW, Payne VDN, Anderson PA, Bhe FT (1979) Immunobiology and species distribition of Mycobacterium tuberculosis antigen 5. Infect Immun 24:77–82

    Google Scholar 

  6. Daniel TM, Debanne SM, Van-der-kuyp F (1985) Enzyme-linked immunosorbent assay, using Mycobacterium tuberculosis antigen 5 and PPD for the serodiagnosis of tuberculosis. Chest 88:388–392

    Google Scholar 

  7. Engvall E, Perlmann P (1972) Enzyme-linked immunosorbent assay. ELISA III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen coated tubes. J Immunol 109:129–135

    Google Scholar 

  8. Hernandez R, Munoz O, Guiscafre H (1984) Sensitive enzyme immunoassay for early diagnosis of tuberculous meningitis. J Clin Microbiol 20:533–535

    Google Scholar 

  9. Janicki BW, Chaparas SD, Daniel TM, Kubica GP, Wright GL, Yee GS (1971) A reference system of Mycobacterium tuberculosis Am Rev Respir Dis 104:602–604

    Google Scholar 

  10. Kalish SB, Radin RC, Levitz D, Zeiss CR, Phair JP (1983) The enzyme-linked immunosorbent method for IgG antibody to purified protein derivative in cerebrospinal fluid of patients with tuberculous meningitis. Ann Intern Med 99:630–633

    Google Scholar 

  11. Laemmli UK (1970) clevage of structural proteins during the assembly of the head of the bacteriophage Nature 227:680–683

    PubMed  Google Scholar 

  12. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folinphenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  13. Ma Y, Wang YM, Daniel TM (1986) Enzyme-linked immunosorbent assay using Mycobacterium tuberculosis antigen 5 for the diagnosis of pulmonary tuberculosis in China. Am Rev Respir Dis 134:1273–1275

    Google Scholar 

  14. Pillai S, Mohimen A (1982) A solid-phases radioimmunoassay for Entamoeba histolytica and detection of circulating antigens in amoebiasis. Gastroenterology 83:1210–1216

    Google Scholar 

  15. Prabhakar S, Oommen A (1987) ELISA using mycobacterial antigens as a diagnostic aid for tuberculous meningitis. J Neurol Sci 78:203–211

    Google Scholar 

  16. Radha Krishnan VV, Mathai A, Rao SB, Sehgel S (1990) Immunoelectrophoresis of mycobacterial antigens. Indian J Exp Biol 28:812–815

    Google Scholar 

  17. Thongkrajai P, Lulitanon V, Chamnavanakit C (1989) Improved ELISA with immunoabsorbent purified mycobacterial antigen for the serodiagnosis of tuberculosis. J Med Microbiol 30:101–104

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology Sree Chitra Tirunal Institute for Medical Sciences and Technology, 695 011, Trivandrum, India

    V. V. Radha Krishnan & A. Mathai

Authors
  1. V. V. Radha Krishnan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Mathai
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Radha Krishnan, V.V., Mathai, A. Isolation of two antigens from the culture filtrates of Mycobacterium tuberculosis and their applications in the laboratory diagnosis of the tuberculous meningitis. Med Microbiol Immunol 180, 101–107 (1991). https://doi.org/10.1007/BF00193851

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00193851

Keywords

Search

Navigation