Summary
A new way of measuring indirect immunofluorescence (IIF) of microscopic bacterial slide preparations by videocamera and an image processing system is presented. This method is compared with the conventional method of reading the slides by eye. The advantages of this new approach are objective reading, greater accuracy and easier performance. We have applied the method to measure serum antibody titres against endogenous Enterobacteriaceae. The method offers the opportunity to combine IIF with automatic morphological analysis, thereby maximally exploiting the possibilities of the immunofluorescence technique.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cohen IR, Norins LC (1966) Natural human antibodies to gram-negative bacteria: immunoglobulins G, A and M. Science 152:1257–1259
Danielsson D, Forsum U (1975) Diagnosis of Neisseria by defined immunofluorescence. Methodologic aspects and applications. Ann N Y Acad Sci 254:334–349
Gonzalez RC, Wintz P (1987) Digital image processing, 2nd edn. Addison Wesley, Reading, pp 139–201, 331–388
Kittler J, Illingworth J, Föglein J (1985) Threshold selection based on a simple image statistic. Computer Vis Graph Image Process 30:125–147
Lebrun L, Pillot J, Grangeot-Keros L, Rannon M-T (1982) Detection of human Fc (gamma) receptors on streptococci by indirect immunofluorescence staining: a survey of streptococci freshly isolated from patients. J Clin Microbiol 16:200–201
Meijer BC, Kootstra GJ, Wilkinson MHF (1990) Theoretical and practical investigation into the characterization of bacterial species by image analysis. Binary 2:21–31
Meijer-Severs GJ, Aarnoudse JG, Mensink WFA, Dankert J (1979) The presence of antibody coated anaerobic bacteria in asymptomatic bacteriuria during pregnancy. J Infect Dis 140:653–658
Monteiro E, Fossey J, Shiner M, Drasar BS, Allison AC (1971) Antibacterial antibodies in rectal and colonic mucosa in ulcerative colitis. Lancet 1:249–251
Nairn RC (1969) In: Fluorescent protein tracing, 3rd edn. E&S Livingstone, Edinburgh London, pp 111–151
Press WH, Flannery BP, Teukolsky SA, Vetterling WT (1986) Numerical recipes. Cambridge University Press, Cambridge, pp 543–546
Schwan A, Ryden A-C (1978) The clinical importance of anaerobic bacteria in wound infections after gastrointestinal surgery. Scand J Infect Dis 10:119–125
Van der Waaij D, Tielemans-Speltie TM, De Roeck-Houben AMJ (1977) Infection by and distribution of biotypes of Enterobacteriaceae species in leukaemic patients treated under ward conditions and in units for protective isolation in seven hospitals in Europe. Infection 3:188–194
Zaren E, Schwan A, Frenckner B (1987) Age related variations of serum concentrations of normally occurring IgG antibodies to Clostridium perfringens. J Clin Pathol 40:282–285
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Apperloo-Renkema, H.Z., Wilkinson, M.H.F., Oenema, D.G. et al. Objective quantitation of serum antibody titres against Enterobacteriaceae using indirect immunofluorescence, read by videocamera and image processing system. Med Microbiol Immunol 180, 93–100 (1991). https://doi.org/10.1007/BF00193850
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00193850