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Identification and characterization of normal length nonfluorescent Y chromosomes: cytogenetic analysis, Southern hybridization and non-isotopic in situ hybridization

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Summary

In two female patients with a 45,X/46,X,+mar karyotype the marker chromosomes were identified as normal length nonfluorescent Y chromosomes (nlYnf) using non-isotopic in situ hybridization (NISH) complementary to routine cytogenetic analysis and Southern hybridization. The recognition of the nlYnf as isodicentric in both patients illustrates and confirms the usefulness and importance of NISH in the identification and characterization of this and many other types of complex chromosome rearrangements.

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Authors and Affiliations

  1. Department of Medical Genetics, University Hospital, De Pintelaan 185, B-9000, Gent, Belgium

    Frank Speleman, Miet Vercruyssen & Jules Leroy

  2. Department of Pediatrics, University Hospital, De Pintelaan 185, B-9000, Gent, Belgium

    Margarita Craen & Jules Leroy

  3. Department of Medical Genetics, University of Antwerp, B-2610, Wilrÿk, Belgium

    Bart Van der Auwera & Kathelijne Mangelschots

  4. Department of Cytochemistry and Cytometry, University of Leiden, NL-2333, AL Leiden, The Netherlands

    Ton Raap & Joop Wiegant

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  1. Frank Speleman
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  2. Bart Van der Auwera
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  3. Kathelijne Mangelschots
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  4. Miet Vercruyssen
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  7. Margarita Craen
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Speleman, F., Van der Auwera, B., Mangelschots, K. et al. Identification and characterization of normal length nonfluorescent Y chromosomes: cytogenetic analysis, Southern hybridization and non-isotopic in situ hybridization. Hum Genet 85, 569–575 (1990). https://doi.org/10.1007/BF00193576

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  • DOI: https://doi.org/10.1007/BF00193576

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