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Induction of anti-pneumococcal cell wall polysaccharide antibodies by type 4 pneumococcal polysaccharide-protein conjugates

Medical Microbiology and Immunology volume 181, pages 35–42 (1992)Cite this article

Abstract

We have prepared polysaccharide-protein conjugates consisting of type 4 pneumococcal capsular polysaccharide (PS4) coupled to tetanus toxoid. The PS4 preparation used contained 2.5% pneumococcal cell wall polysaccharide (CPs). During the conjugation process, in addition to PS4-protein conjugates, CPs-protein conjugates were also formed. After immunization with PS4-protein conjugates, CPs-protein conjugates that are present as a contaminant induce IgG anti-CPs antibodies in mice. Pneumococcal oligosaccharides, prepared by periodate oxidation of PS4, did not contain detectable amounts of CPs; hence, oligosaccharide-protein conjugates did not induce anti-CPs antibodies.

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Affiliations

  1. Department of Immunology, Univeristy Hospital for Children and Youth “Het Wilhelmina Kinderziekenhuis”, State University, School of Medicine, Utrecht, The Netherlands

    Carla Peeters, Anne-Marie Tenbergen-Meekes, Ben Zegers & Ger Rijkers

  2. Unit for Bacterial Vaccine Development and Pathogenesis Research, National Institute of Public Health and Environmental Protection, Antonie van Leeuwenhoeklaan 9, P.O. Box 1, NL-3720, BA Bilthoven, The Netherlands

    Carla Peeters & Jan Poolmann

Authors
  1. Carla Peeters
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  2. Anne-Marie Tenbergen-Meekes
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  3. Jan Poolmann
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  4. Ben Zegers
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  5. Ger Rijkers
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Peeters, C., Tenbergen-Meekes, AM., Poolmann, J. et al. Induction of anti-pneumococcal cell wall polysaccharide antibodies by type 4 pneumococcal polysaccharide-protein conjugates. Med Microbiol Immunol 181, 35–42 (1992). https://doi.org/10.1007/BF00193394

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  • DOI: https://doi.org/10.1007/BF00193394

Keywords

  • Cell Wall
  • Polysaccharide
  • Oligosaccharide
  • Tetanus
  • Periodate