Abstract
Feeding experiments with tritium- and deuterium-labeled castasterone (CS) were conducted with three cell lines of Catharanthus roseus, including crown gall cells and nontransformed cells. In all three cell lines, the conversion of CS to brassinolide (BL) was observed and unequivocally confirmed by gas chromatography/mass spectrometry (GC/MS). This is the first conclusive evidence that CS is the biosynthetic precursor of BL.
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Biosynthesis of brassinosteroids in Catharanthus roseus. Part II. Part I of this series: Yokota et al. (1990a).
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Suzuki, H., Fujioka, S., Takatsuto, S. et al. Biosynthesis of brassinolide from castasterone in cultured cells of Catharanthus roseus . J Plant Growth Regul 12, 101 (1993). https://doi.org/10.1007/BF00193241
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DOI: https://doi.org/10.1007/BF00193241