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Improved fragile site detection with trimethoprim

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Summary

The effect of trimethoprim, an inhibitor of dihydrofolate reductase, in demonstrating the presence of fragile sites on human chromosomes was investigated. Lymphocyte cultures with 20 mg/l trimethoprim added at the onset of incubation gave consistently higher frequencies of fragile sites than the other culture regimes tested.

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Authors and Affiliations

  1. Human Genetics Laboratory, Department of Pathology, University of Dundee, Ninewells Hospital, DD1 9SY, Dundee, UK

    Doris A. McLean & Michael J. W. Faed

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  1. Doris A. McLean
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  2. Michael J. W. Faed
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McLean, D.A., Faed, M.J.W. Improved fragile site detection with trimethoprim. Hum Genet 85, 241–243 (1990). https://doi.org/10.1007/BF00193204

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  • DOI: https://doi.org/10.1007/BF00193204

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