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Medicarpin and maackiain 3-O-glucoside-6′-O-malonate conjugates are constitutive compounds in chickpea (Cicer arietinum L.) cell cultures

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Summary

The pterocarpan phytoalexin conjugates medicarpin 3-O-glucoside-6′-O-malonate and maackiain 3-O-glucoside-6′-O-malonate were isolated from cell suspension cultures of chickpea (Cicer arietinum L.) cultivar ILC 3279 and structurally elucidated. Both pterocarpan conjugates are constitutive metabolites of the chickpea cell cultures. Upon application of an elicitor from yeast to the cell cultures a substantial increase in the level of the phytoalexin aglycones medicarpin and maackiain was observed although a delayed but significantly higher rise of the conjugates also occurred. The significance of the pterocarpan conjugates for phytoalexin production is discussed.

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Abbreviations

MeGM:

medicarpin 3-O-glucoside-6′-O-malonate

MaGM:

maackiain 3-O-glucoside-6′-O-malonate

MeG:

medicarpin 3-O-glucoside

MaG:

maackiain 3-O-glucoside

FGM:

formononetin 7-O-glucoside-6″-O-malonate

BGM:

biochanin A 7-O-glucoside-6″-O-malonate

IFR:

NADPH: 2′-hydroxyisoflavone oxidoreductase

PTS:

pterocarpan synthase

IGT:

UDP-glucose: isoflavone 7-O-glucosyltransferase

IMT:

malonyl-coA: isoflavone 7-O-glucoside-6″ -O-malonyltransferase

RT:

retention time

sh:

shoulder

d :

duplette

m :

multiplette

s :

singulette

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Authors and Affiliations

  1. Institut für Biochemie und Biotechnologie der Pflanzen, Westfälische Wilhelms-Universität, W-4400, Münster, Germany

    C. Weidemann, R. Tenhaken, U. Höhl & W. Barz

Authors
  1. C. Weidemann
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  2. R. Tenhaken
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  3. U. Höhl
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  4. W. Barz
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Communicated by E. W. Weiler

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Weidemann, C., Tenhaken, R., Höhl, U. et al. Medicarpin and maackiain 3-O-glucoside-6′-O-malonate conjugates are constitutive compounds in chickpea (Cicer arietinum L.) cell cultures. Plant Cell Reports 10, 371–374 (1991). https://doi.org/10.1007/BF00193162

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  • DOI: https://doi.org/10.1007/BF00193162

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