Summary
We have established a unique betalain pigmentation system in callus cultures that originated from seedlings of Portulaca sp. ‘Jewel’. Within three different ‘Jewel’ lines examined, one line (JR) was clearly superior with regard to callus growth rate and pigment formation. Furthermore, after ten cycles of selection of deeply colored callus patches, the selected clones contained on an average four times the amount of betalain as compared to the non-selected mother line. The colorization was induced by light, but disappeared in the dark. Pigment synthesis was detectable within 30 h after irradiation and showed positive correlation with irradiation periods.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxy-acetic acid
- HPLC:
-
high performance liquid chromatography
References
Adachi T, Nakatsukasa M (1983) Z. Pflanzenphysiol. 109:155–162
Adachi T, Nakatsukasa M, Asaka Y, Uta T (1985) Japan. J. Breed. 35:183–192
Berlin J, Sieg S, Strack D, Bokern M, Harms H (1986) Plant Cell, Tissue and Organ Cult. 5:163–174
Boehm H, Rink E (1988) In: Constabel F, Vasil IK (eds) Cell culture and somatic cell genetics of plants. Vol 5, Academic Press, pp 449–463
Constabel F, Nassif-Makki H (1971) Ber. Dtsch. Bot. Gas. 84:629–636
Elliott DC (1983) J. Exp. Bot. 34:67–73
Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50:151–158
Girod PA, Zryd JP, (1986) Experientia 42:653–654
Girod PA, Zryd JP, (1987) Plant Cell Rep. 6:27–30
Giudici de Nicola M, Piattelli M, Amico V (1973) Phytochemistry 12:2163–2166
Giudici de Nicola M, Amico V, Piattelli M (1974) Phytochemistry 13:439–442
Hashimoto T, Matsuda J, Okabe S, Amano Y, Yun DJ, Hayashi A, Yamada Y (1990) In: Nijkamp HJJ, van der Plas LHW, van Aartrijk J (eds) Progress in plant cellular and molecular biology, Kluwer Academic Publishers, pp 775–780
Hirose M, Yamakawa T, Kodama T, Komamine A (1990) Plant Cell Physiol. 31:267–271
Koehler KH (1972) Phytochemistry 11:127–131
Kumon K, Sasaki J, Sejima M, Hayashi Y, Takeuchi Y (1990) Plant Cell Physiol. 31:391–393
Kuchan TM, Hampp N, Lottspeich F, Beyreuther K, Zenk MH (1988) FEBS Lett. 237:40–44
Liebisch HW, Boehm H (1981) Pharmazie 36:218
Luhrs R, Loerz H (1987) Theor. Appl. Genet. 75:16–25
Martin CR, Carpenter R, Coen ES, Gerats AGM (1987) In: Thomas H, Grierson D (eds) Developmental mutants in higher plants, Cambridge University Press, pp 19–52
Meijer AH, Pennings EJM, de Waal A, Verpoorte R (1990) In: Nijkamp HJJ, van der Plas LHW, van Aartrijk J (eds) Progress in plant cellular and molecular biology, Kluwer Academic Publishers, pp 769–774
Mikami T, Kinoshita T (1988) Plant Cell, Tissue and Organ Cult. 12:311–314
Mol JNM, Stuitje AR, van der Krol A (1989) Plant Mol. Biol. 13:287–294
Murashige T, Skoog F (1962) Physiol. Plant. 15:473–497
Okada N, Koizumi N, Tanaka T, Ohkubo H, Nakanishi S, Yamada Y (1989) Proc. Natl. Acad. Sci. USA 86:534–538
Sakuta M, Takagi T, Komamine A (1986) J. Plant Physiol. 125:337–343
Sakuta M, Takagi T, Komamine A (1987a) Physiol. Plant. 71:455–458
Sakuta M, Takagi T, Komamine A (1987b) Physiol. Plant. 71:459–463
Schulte U, El-Shagi H, Zenk MH (1984) Plant Cell Rep. 3:51–54
Syakudo K, Kawabata S, Ujihara A (1960) Jap. J. Genet. 35:107–109
Vasil IK (1988) Bio/technology 6:397–402
Yamamoto Y, Mizuguchi R, Yamada Y (1982) Theor. Appl. Genet. 61:113–116
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Kishima, Y., Nozaki, K., Akashi, R. et al. Light-inducible pigmentation in Portulaca callus; selection of a high betalain producing cell line. Plant Cell Reports 10, 304–307 (1991). https://doi.org/10.1007/BF00193147
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DOI: https://doi.org/10.1007/BF00193147