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Histological and immunopathological analysis of T-cells mediating murine HSV-1 keratitis

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Abstract

• Background: Thymusderived lymphocytes play a critical role in the development of herpes simplex keratitis (HSK). T-cell subsets defined by their expression of various T-cell receptor (TCR) Vß segments were studied following corneal HSV-1 infection (p.i.). • Methods: Conjunctiva, corneal limbus and corneal stroma of two inbred BALB/c congenic mouse strains which differ only in the gene products closely linked to the Igh-1 locus on chromosome 12 were analyzed. • Results: While C.B-17 mice (Igh-1b) were resistant to HSK, C.AL-20 mice (Igh-1d) clinically developed severe necrotizing keratitis by day 11 p.i. The corneal stroma of C.B-17 mice remained clear, while it was increasingly infiltrated by mononuclear cells and neutrophils in C.AL-20 mice by day 11 p.i. In C.B-17 mice, Thy1.2+ cells were found in the conjunctiva between days 2 to 4 p.i., and subsequently decreased. Only a few Thyl.2+ cells were found in the limbus, and no such cells were found in the stroma. In contrast, in C.AL-20 mice the numbers of Thyl.2+ cells (activated CD4+, Vß8+ T cells) profoundly increased in the conjunctiva by day 4 p.i. These cells infiltrated the limbus between days 7 and 11 p.i. and eventually entered the stromal tissue by day 11 p.i. • Conclusions: Our data suggest that the HSV-1-induced corneal tissue destruction is mediated by mononuclear cells and neutrophils and that these cells are probably attracted into the cornea by cytokines elaborated by activated CD4+, Vß8+ T cells.

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Author notes

  1. Arnd Heiligenhaus

    Present address: Department of Ophthalmology, University of Essen, Hufelandstrasse 55, D-45122, Essen, Germany

Authors and Affiliations

  1. Hilles Immunology Laboratory, Massachusetts Eye & Ear Infirmary, Harvard Medical School, 243 Charles Street, 02114, Boston, MA, USA

    Arnd Heiligenhaus & C. Stephen Foster

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  1. Arnd Heiligenhaus
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  2. C. Stephen Foster
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Presented as a paper at the ECORA Meeting, 4–7 October 1993, Bonn

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Heiligenhaus, A., Foster, C.S. Histological and immunopathological analysis of T-cells mediating murine HSV-1 keratitis. Graefe's Arch Clin Exp Ophthalmol 232, 628–634 (1994). https://doi.org/10.1007/BF00193124

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  • DOI: https://doi.org/10.1007/BF00193124

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