References
Berg PA, Klein R (1988) Molecular determination of the primary biliary cirrhosis-specific M2 antigen. Hepatology 8: 200
Berg PA, Doniach D, Roitt IM (1967) Mitochondrial antibodies in primary biliary cirrhosis. 1. Localisation of the antigen to mitochondrial membranes. J Exp Med 126: 277
Berg PA, Klein R, Lindenborn-Fotinos J, Kloppel W (1982) ATPase-associated antigen (M2): marker antigen for serological diagnosis of primary biliary cirrhosis. Lancet II: 1423
Berg PA, Klein R, Lindenborn-Fotinos J (1986) Antimitochondrial antibodies in primary biliary cirrhosis. J Hepatol 2: 123
Bradford AP, Howell S, Aitken A, James LA, Yeaman SJ (1987) Primary structure around the lipoate attachment site on the E2 component of bovine heart pyruvate dehydrogenase complex. Biochem J 245: 919
Baum H, Palmer C (1985) The PBC-specific antigen. Mol Aspects Med 8: 201
Coppel RL, McNeilage LJ, Surh CD, Van de Water J, Spithill TW, Whittingham S, Gershwin ME (1988) Primary structure of the human M2 mitochondrial autoantigen of primary biliary cirrhosis: dihydrolipoamide acetyltransferase. Proc Natl Acad Sci USA 85: 7317
Crowe JP, Christensen E, Butler J, Wheeler D, Doniach D, Keenan J, Williams R (1980) Primary biliary cirrhosis: the prevalence of hypothyroidism and its relationship to thyroid autoantibodies and sicca syndrome. Gastroenterology 78: 1437
De Marcucci O, Lindsay JG (1985) Component X, an immunologically distinct polypeptide associated with mammalian pyruvate dehydrogenase multi-enzyme complex. Eur J Biochem 149: 641
Doniach D, Roitt IM, Walker JG, Sherlock S (1966) Tissue antibodies in primary biliary cirrhosis, active chronic (lupoid) hepatitis, cryptogenic cirrhosis and other liver diseases and their clinical implications. Clin Exp Immunol 1: 237
Frazer IH, Mackay IR, Jordan TW, Whittingham S, Marzuki S (1985) Reactivity of antimitochondrial antibodies in primary biliary cirrhosis: further biochemical and immunological characterisation of mitochondrial antigens. J Immunol 135: 1739
Fregeau DR, Davis PA, Danner DJ, Ansari A, Coppel RL, Dickson ER, Gershwin ME (1989) Antimitochondrial antibodies of primary biliary cirrhosis recognise dihydrolipoamide acyltransferase and inhibit enzyme function of the branched chain-α-keto acid dehydrogenase complex. J Immunol 142: 3815
Fusconi M, Ghadiminejad I, Bianchi FB, Baum H, Bottazzo GF, Pisi E (1988) Heterogeneity of antimitochondrial antibodies with the M2-M4 pattern by immunofluorescence as assessed by western blotting and enzyme linked immunosorbent assay. Gut 29: 440
Fussey SPM, Guest JR, James OFW, Bassendine MF, Yeaman SJ (1988) Identification and analysis of the major M2 autoantigens in primary biliary cirrhosis. Proc Natl Acad Sci USA 85: 8654
Fussey SPM, Bassendine MF, James OFW, Yeaman SJ (1989) Characterisation of the reactivity of autoantibodies in primary biliary cirrhosis. FEBS Lett 246: 49
Fussey SPM, Bassendine MF, Fittes D, Turner IB, James OFW, Yeaman SJ (1989) The E1α and β subunits of the pyruvate dehydrogenase complex are M2“d” and M2“e” autoantigens in primary biliary cirrhosis. Clin Sci 77: 365
Gershwin ME, Mackay IR, Sturgess A, Coppel RL (1987) Identification and specificity of a cDNA encoding the 70-kDa mitochondrial antigen recognised in primary biliary cirrhosis. J Immunol 138: 3525
Gershwin ME, Coppel RL, Mackay IR (1988) Primary biliary cirrhosis and mitochondrial autoantigens — insights from molecular biology. Hepatology 8: 147
Ghadiminejad I, Baum H (1987) Evidence for the cell-surface localisation of antigens cross-reacting with the “mitochondrial antibodies” of primary biliary cirrhosis. Hepatology 7: 743
Hodgson JA, De Marcucci OG, Lindsay JG (1986) Lipoic acid is the site of substrate-dependent acetylation of component X in ox heart pyruvate dehydrogenase multienzyme complex. Eur J Biochem 158: 595
Hummel KB, Litwer S, Bradford AP, Aitken A, Danner DJ, Yeaman SJ (1988) Nucleotide sequence of a cDNA for branched-chain acyltransferase with analysis of deduced protein structure. J Biol Chem 263: 6165
Ishii H, Saifuku K, Namihisa T (1985) Multiplicity of mitochondrial inner membrane antigens from beef heart reacting with antimitochondrial antibodies in sera of patients with primary biliary cirrhosis. Immunol Lett 9: 325
Ishii H, Saifuku K, Namihisa T (1987) Reactivities and clinical relevance of antimitochondrial antibodies to four mitochondrial inner membrane proteins in sera of patients with primary biliary cirrhosis. Hepatology 7: 134
Jilka JM, Rahmatullah M, Kazemi M, Roche TE (1986) Properties of a newly characterised protein of the bovine kidney pyruvate dehydrogenase complex. J Biol Chem 261: 1858
Kerscher L, Oesterhelt D (1982) Pyruvate: ferridoxin oxidoreductase — new findings on an ancient enzyme. Trends Biochem Sci 7: 371
Klein R, Berg P-A (1988) Characterisation of a new mitochondrial antigen-antibody system (M9/anti-M9) in patients with anti M2-positive and anti-M2-negative primary biliary cirrhosis. Clin Exp Immunol 74: 68
Koike K, Ohta S, Urata Y, Kagawa Y, Koike M (1988) Cloning and sequencing of cDNAs encoding α and β subunits of human pyruvate dehydrogenase. Proc Natl Acad Sci USA 85: 41
Kyriatsoulis A, Manns M, Gerken G, Lohse AW, Maelicke A, Wessler I, Reske K, Meyer-zum-Buschenfelde K-H (1988) Immunochemical characterisation of anti-acetylcholine receptor antibodies in primary biliary cirrhosis. J Hepatol 6: 283
Lassoued K, Guilly M-N, Andre C, Paintrand M, Dhumeaux D, Danon F, Brouet JC, Courvalin JC (1988) Autoantibodies to 200-kDa polypeptide(s) of the nuclear envelope: a new serological marker of primary biliary cirrhosis. Clin Exp Immunol 74: 283
Lindenborn-Fotinos J, Sayers TJ, Berg PA (1982) Mitochondrial antibodies in primary biliary cirrhosis. VI. Association of the complement-fixing antigen with a component of the mitochondrial F1-ATPase complex. Clin Exp Immunol 50: 267
Lindenborn-Fotinos J, Baum H, Berg PA (1985) Mitochondrial antibodies in primary biliary cirrhosis. Further characterisation of the M2 antigen by immunoblotting revealing species and non-species specific determinants. Hepatology 5: 763
Mackay IR (1958) Primary biliary cirrhosis showing high titre of autoantibody: report of a case. N Engl J Med 258: 185
Mendel-Hartvig I, Nelson BD, Loof L, Totterman TH (1985) Primary biliary cirrhosis: further biochemical and immunological characterisation of mitochondrial antigens. Clin Exp Immunol 62: 371
Miles JS, Guest JR (1987) Molecular genetic aspects of the citric acid cycle of Escherichia coli. Biochem Soc Symp 54: 45
Mutimer DJ, Fussey SPM, Yeaman SJ, Kelly PJ, James OFW, Bassendine MF (1989) Frequency of IgG and IgM autoantibodies to four specific M2 mitochondrial autoantigens in primary biliary cirrhosis. Hepatology 10: 403
Ohmori H, Yamauchi T, Yamamoto I (1986) Augmentation of the antibody response by lipoic acid in mice, (ii) restoration of the antibody response in immunosuppressed mice. Japan J Pharmacol 42: 275
Pons G, Raefsky-Estrin C, Carothers PJ, Pepin PA, Javed AA, Jesse BW, Ganapathi MK, Samols D, Patel MS (1988) Cloning and cDNA sequence of the dihydrolipoamide dehydrogenase component of human α-keto acid dehydrogenase complexes. Proc Natl Acad Sci USA 85: 1422
Ruffati A, Arslan P, Floreani A, De Silvestro G, Calligaro A, Naccarato R, Todesco S (1985) Nuclear membrane staining antinuclear antibody in patients with primary biliary cirrhosis. J Clin Immunol 5: 357
Schultheiss HP, Berg PA, Klingenberg M (1983) The mitochondrial adenine nucleotide translocator is an antigen in primary biliary cirrhosis. Clin Exp Immunol 54: 648
Stemerowicz R, Hopf U, Moller B, Wittenbrink C, Rodloff A, Reinhardt R, Freudenberg M, Galanos C (1988) Are mitochondrial antibodies in primary biliary cirrhosis induced by R(rough)-mutants of enterobacteriaceae. Lancet II: 1166
Surh CD, Danner DJ, Ahmed A, Coppel RL, Mackay IR, Dickson ER, Gershwin ME (1989) Reactivity of primary biliary cirrhosis sera with a human fetal liver cDNA clone of branched-chain α-keto acid dehydrogenase dihydrolipoamide acyltransferase, the 52 kDa mitochondrial autoantigen. Hepatology 9: 63
Surh CD, Roche TE, Danner DJ, Ansari A, Coppel RL, Dickson R, Gershwin ME (1989) Anti-mitochondrial autoantibodies in primary biliary cirrhosis recognise cross-reactive epitope(s) on protein X and dihydrolipoamide acetyltransferase of pyruvate dehydrogenase complex. Hepatology 10: 127
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350
Tzartos SJ, Lindstrom JM (1980) Monoclonal antibodies used to probe acetylcholine receptor structure: localisation of the main immunogenic region and detection of similarities between subunits. Proc Natl Acad Sci USA 77: 755
Van de Water J, Fregeau D, Davis P, Ansari A, Danner D, Leung P, Coppel R, Gershwin ME (1988) Autoantibodies of primary biliary cirrhosis recognise dihydrolipoamide acetyltransferase and inhibit enzyme function. J Immunol 141: 2321
Van de Water J, Gershwin ME, Leung P, Ansari A, Coppel RL (1988) The autoepitope of the 74-kDa mitochondrial autoantigen of primary biliary cirrhosis corresponds to the functional site of dihydrolipoamide acetyltransferase. J Exp Med 167: 1791
Yeaman SJ (1986) The mammalian 2-oxo-acid dehydrogenases: a complex family. Trends Biochem Sci 11: 293–296
Yeaman SJ (1989) The 2-oxo-acid dehydrogenase complexes: recent advances. Biochem J 257: 625
Yeaman SJ, Fussey SPM, Danner DJ, James OFW, Mutimer DJ, Bassendine MF (1988) Primary biliary cirrhosis: identification of two major M2 mitochondrial autoantigens. Lancet I: 1067
Yeaman SJ, Fussey SPM, Mutimer DJ, James OFW, Bassendine MF (1989) M2 autoantigens in Primary Biliary Cirrhosis. Lancet I: 103
Walker JG, Doniach D, Roitt IM, Sherlock S (1965) Serological tests in the diagnosis of primary biliary cirrhosis. Lancet I: 827
Weber P, Brenner J, Stechemesser E, Klein R, Weckenham U, Kloppel G, Kirckhof M, Fintelmann V, Berg PA (1986) Characterisation and clinical relevance of a new complement-fixing antibody-anti-M8 —in patients with primary biliary cirrhosis. Hepatology 6: 553
Zhang B, Crabb DW, Harris RA (1988) Nucleotide and deduced amino acid sequence of the E1α subunit of human liver branched-chain α-keto acid dehydrogenase. Gene 69: 159
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Bassendine, M.F., Yeaman, S.J. Primary biliary cirrhosis: Nature of autoantigens. Springer Semin Immunopathol 12, 73–83 (1990). https://doi.org/10.1007/BF00192684
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DOI: https://doi.org/10.1007/BF00192684