Diagnosis of tuberculous meningitis by enzyme-linked immunosorbent assay to detect mycobacterial antigen and antibody in cerebrospinal fluid

Abstract

Enzymed-linked immunosorbent assay (ELISA) was used to detect mycobacterial antigen and antimycobacterial antibody in cerebrospinal fluid (CSF) specimens of 50 patients with tuberculous meningitis (TBM) and 50 patients with non-tuberculous neurological diseases (control group). The assay gave no false negative results in 10 culture-positive patients with TBM. Detection of mycobacterial antigen in CSF is more sensitive and specific for the diagnosis of TBM than detection of antibody. ELISA should be considered as one of the alternative methods in the laboratory diagnosis of TBM, particularly in culturenegative patients with TBM.

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References

  1. 1.

    Bal V, Kamat RS, Kamat J, Kandoth P (1983) Enzyme-linked immunosorbent assay for mycobacterial antigens. Indian J Med Res 78:477–483

    Google Scholar 

  2. 2.

    Chandramukhi A, Allen PRJ, Ken M, Ivanyi J (1985) Detection of mycobacterial antigens and antibodies in the cerebrospinal fluid in patients with tuberculous meningitis. J Med Microbiol 20:239–247

    Google Scholar 

  3. 3.

    Chandramukhi A, Bothamley GH, Brennan PJ, Ivanyi J (1989) Levels of antibody to defined antigens of Mycobacterium tuberculosis in tuberculous meningitis. J Clin Microbiol 27:821–825

    Google Scholar 

  4. 4.

    Coovadia YM, Dawood A, Ellis ME, Coovadia HM, Daniel TM (1986) Evaluation of adenosine deaminase activity and antibody to Mycobacterium tuberculosis antigen-5 in cerebrospinal fluid and the radioactive bromide-partition test for the early diagnosis of tuberculous meningitis. Arch Dis Child 61:428–435

    Google Scholar 

  5. 5.

    Engvall E, Perlmann P (1972) Enzyme-linked immunosorbent assay, ELISA III Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. J Immunol 109:129–135

    Google Scholar 

  6. 6.

    Hernandez R, Munoz O, Guiscafre H (1984) Sensitive enzyme-immunoassay for early diagnosis of tuberculous meningitis J Clin Microbiol 20:533–535

    Google Scholar 

  7. 7.

    Kadival GV, Samuel AM, Virdi BS, Kala RN, Ganatra RD (1982) Radioimmunoassay of tuberculous antigen. Indian J Med Res 75:765–770

    Google Scholar 

  8. 8.

    Kalish SB, Radin RC, Levitz D, Zeiss CR, Phair JP (1983) The enzyme-linked immunosorbent assay method for IgG antibody to purified protein derivative in cerebrospinal fluid of patients with tuberculous meningitis. Ann Intern Med 99:630–633

    Google Scholar 

  9. 9.

    Molavi A, Le Frock JL (1985) Tuberculous meningitis. Med Clin N Am 69:315–331

    Google Scholar 

  10. 10.

    Prabhakar S, Oommen A (1987) ELISA using mycobacterial antigen as a diagnostic aid for tuberculous meningitis. J Neurol Sci 78:203–211

    Google Scholar 

  11. 11.

    Virmani V, Geetha R, Shrinivas G (1975) A study of cerebrospinal fluids in atypical presentation of tuberculous meningitis. J Neurol Sci 26:587–592

    Google Scholar 

  12. 12.

    Voller A, Bidwell DE, Bartlett A (1976) Enzyme immuno-assays in diagnostic medicine. Bull WHO 53:53–65

    Google Scholar 

  13. 13.

    Watt G, Zaraspe G, Bautista S, Laughlin LW (1988) Rapid diagnosis of tuberculous meningitis by using an enzyme-linked immunosorbent assay to detect mycobacterial antigen and antibody in cerebrospinal fluid J Infect Dis 158:681–686

    Google Scholar 

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Correspondence to V. V. Radhakrishnan.

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Annamma, M., Radhakrishnan, V.V. & Shobha, S. Diagnosis of tuberculous meningitis by enzyme-linked immunosorbent assay to detect mycobacterial antigen and antibody in cerebrospinal fluid. Med Microbiol Immunol 179, 281–288 (1990). https://doi.org/10.1007/BF00192466

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Keywords

  • Meningitis
  • Cerebrospinal Fluid
  • Immunosorbent Assay
  • Neurological Disease
  • False Negative Result