Summary
When mature pollen grains of Spinacia oleracea were squashed in a 25% sucrose solution and subsequently centrifuged on a percoll layer, sperm cells were isolated in high numbers. All steps were carried out at 4° C. Isolated sperm cells could be kept alive for several hours.
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Theunis, C.H., Van Went, J.L. Isolation of sperm cells from mature pollen grains of Spinacia oleracea L.. Sexual Plant Reprod 2, 97–102 (1989). https://doi.org/10.1007/BF00191997
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DOI: https://doi.org/10.1007/BF00191997