Summary
Maize pollen was exposed to the herbicide Chlorsulfuron (CS), and segregation for tolerance was observed. The resulting plant generation exhibited significantly greater tolerance to CS than other (control) progeny. Since the increase in tolerance occurred after only one generation of pollen exposure, this result demonstrates that pollen selection can be used to develop herbicide-resistant crop species, even when the species are not amenable to cell culture. It also suggests a possible mechanism for the rapid evolution of herbicide tolerance in weeds.
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Gorla, M.S., Ottaviano, E., Frascaroli, E. et al. Herbicide-tolerant corn by pollen selection. Sexual Plant Reprod 2, 65–69 (1989). https://doi.org/10.1007/BF00191992
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DOI: https://doi.org/10.1007/BF00191992