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Serodiagnostic efficiency of phospholipid associated protein of Mycobacterium tuberculosis H37Rv

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Abstract

The phospholipid-associated protein (55–67 kDa) fraction of Mycobacterium tuberculosis H37Rv was purified as the DE-V protein fraction. This DE-V fraction was used for diagnosis of tuberculosis by enzyme-linked immunosorbent assay (ELISA), detecting IgG antibody in sera collected from different categories of tuberculosis patients, i.e. with acid fast bacilli (AFB) culture-positive pulmonary tuberculosis, with AFB culture-negative, but radiologically suspected, pulmonary tuberculosis, extrapulmonary tuberculosis, and control groups of patients suffering from diseases other than tuberculosis (asthma and/or rhinitis, lepromatous leprosy) as well as from healthy volunteers. Encouraging operational ELISA validity could be achieved with 93% sensitivity, 100% specificity, 97% efficiency, 100% positive predictivity and 95% negative predictability even among the extrapulmonary and suspected pulmonary tuberculosis patients. The above assay was insensitive but with 100% specificity among control group of patients suffering from diseases other than tuberculosis. The DE-V protein fraction was associated with phosphatidyl inositol and phosphatidyl inositol mannosides. The dissociation of phospholipid-protein complex decreased ELISA specificity. ELISA reactivity of the DE-V fraction appeared to be thermostable; thus, it may have serodiagnostic utility in developing countries.

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Authors and Affiliations

  1. CSIR Centre for Biochemicals, Delhi University Campus, Mall Road, 110007, Delhi, India

    N. K. Kaushik, Parul Sharma & T. A. Venkitasubramanian

  2. Department of Clinical Research, V. P. Chest Institute, Delhi University, 110007, Delhi, India

    Ashok Shah

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  1. N. K. Kaushik
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  2. Parul Sharma
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  3. Ashok Shah
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  4. T. A. Venkitasubramanian
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Kaushik, N.K., Sharma, P., Shah, A. et al. Serodiagnostic efficiency of phospholipid associated protein of Mycobacterium tuberculosis H37Rv. Med Microbiol Immunol 182, 317–327 (1993). https://doi.org/10.1007/BF00191947

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  • DOI: https://doi.org/10.1007/BF00191947

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