Abstract
A three-allele polymorphic system is detectable by direct electrophoretic analysis of the amplified 5′ untranslated und 5′ flanking regions of the HLA-linked HSP70-1 gene. Single nucleotide differences at two sites, i.e., − 110 and + 120, are responsible for changes in the bending pattern and, consequently, in the electrophoretic mobility of the three alleles. The presence of the sequence AAACCCC around position − 110 is strongly associated with DR3.
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Cascino, I., Sorrentino, R. & Tosi, R. Strong genetic association between HLA-DR3 and a polymorphic variation in the regulatory region of the HSP70-1 gene. Immunogenetics 37, 177–182 (1993). https://doi.org/10.1007/BF00191882
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DOI: https://doi.org/10.1007/BF00191882