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Planta

, Volume 197, Issue 4, pp 613–618 | Cite as

Evidence for the involvement of plasma-membrane-bound nitrate reductase in signal transduction during blue-light stimulation of nitrate uptake in Chlorella saccharophila

  • Christine Stöhr
  • Ulrich Glogau
  • Michael Mätschke
  • Rudolf Tischner
Article

Abstract

Nitrate uptake in Chlorella saccharophila (Krüger) Nadson was found to be stimulated by blue light, leading to a doubling of the rate. In the presence of background red light (300 μmol photons · m-2 · s-1), only 15–20 μmol photons · m-2 · s-1 of blue light was sufficient to saturate this increased uptake rate. Incubation of Chlorella cells with anti-nitrate-reductase immunoglobulin-G fragments inhibited blue-light stimulation. However, ferricyanide (10 μM) doubled and dithiothreitol (100 μM) inhibited the stimulatory effect of blue light. Among the protein-kinase inhibitors used, only staurosporine (10 μM) prevented the blue-light stimulation. Phosphatase inhibitors were without effect and sodium vanadate totally inhibited nitrate uptake, pointing to an involvement of the plasma-membrane ATPase. Preincubation of the cells with calmodulin antagonists or calcium ionophores did not significantly reduce blue-light stimulation of nitrate uptake. The data are discussed with regard to transduction of the signal for blue-light stimulation of nitrate uptake and the possibility that the plasma-membrane-bound nitrate reductase is the blue-light receptor.

Key words

Blue-light Chlorella Nitrate reductase (plasma-membrane-bound) Nitrate uptake 

Abbreviations

Chl

chlorophyll

DMSO

dimethylsulfoxide

1,2-DHG

1,2-dihexanoylglycerol

ML-9

1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine

NR

nitrate reductase

H-7

1-(5-isoquinolinyl-sulfonyl)-2-methylpiperazine

IgG

immunoglobulin G

PFD

photon flux density

PM

plasma membrane

W-7

N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Christine Stöhr
    • 1
  • Ulrich Glogau
    • 2
  • Michael Mätschke
    • 2
  • Rudolf Tischner
    • 2
  1. 1.Institut für Botanik, Technische HochschuleDarmstadtGermany
  2. 2.Pflanzenphysiologisches Institut der Universität GöttingenGöttingenGermany

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